Related Experiment Video
Updated: Feb 5, 2026
07:36
An Experimental Analysis of Children's Ability to Provide a False Report about a Crime
Published on: May 3, 2016
9.1K
An empirical analysis of enzyme function reporting for experimental reproducibility: Missing/incomplete information
Peter Halling1, Paul F Fitzpatrick2, Frank M Raushel3
1WestCHEM, Department of P & A Chemistry, University of Strathclyde, Glasgow G1 1XL, United Kingdom.
Biophysical Chemistry
|September 9, 2018
Summary
Enzyme function data reporting lacks critical information for reproducibility in many studies. Implementing systems like STRENDA DB can help capture essential metadata, improving scientific record integrity.
Area of Science:
- Biochemistry
- Enzymology
- Scientific Reproducibility
Background:
- Enzyme function experiments require comprehensive metadata for replication and interpretation.
- Incomplete reporting hinders the full utilization of scientific findings.
Purpose of the Study:
- To evaluate the completeness of enzyme function data reporting for experimental reproducibility.
- To identify common omissions in published enzyme function studies.
Main Methods:
- Detailed examination of 11 recent papers and supplementary materials from leading journals.
- Analysis of 100 papers utilized by the SABIO-RK database.
Main Results:
- Critical information necessary for reproducing enzyme function findings was missing in every examined paper.
- Common omissions include enzyme/substrate concentrations and buffer counter-ion identity.
- Many identified omissions could be captured by the STRENDA DB system.
Conclusions:
- Current enzyme function data reporting is insufficient for ensuring reproducibility.
- Computerized systems like STRENDA DB can improve metadata completeness and secure the scientific record.
Related Concept Videos
01:43Incomplete Dominance
30.0K
Gregor Mendel's work (1822 - 1884) was primarily focused on pea plants. Through his initial experiments, he determined that every gene in a diploid cell has two variants called alleles inherited from each parent. He suggested that amongst these two alleles, one allele is dominant in character and the other recessive. The combination of alleles determines the phenotype of a gene in an organism.
30.0K
02:37Experimental Determination of Chemical Formula
47.4K
The elemental makeup of a compound defines its chemical identity, and chemical formulas are the most concise way of representing this elemental makeup. When a compound’s formula is unknown, measuring the mass of its constituent elements is often the first step in determining the formula experimentally.
47.4K
02:34Enzymes
94.9K
Inside living organisms, enzymes act as catalysts for many biochemical reactions involved in cellular metabolism. The role of enzymes is to reduce the activation energies of biochemical reactions by forming complexes with its substrates. The lowering of activation energies favor an increase in the rates of biochemical reactions.
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
94.9K
01:09Empirical Method to Interpret Standard Deviation
10.2K
The empirical rule, also known as the three-sigma rule, allows a statistician to interpret the standard deviation in a normally distributed dataset. The rule states that 68% of the data lies within one standard deviation from the mean, 95% lies within two standard deviations from the mean, and 99.7% lies within three standard deviations from the mean. Additionally, this rule is also called the 68-95-99.7 rule.
This rule is used widely in statistics to calculate the proportion of data values...
This rule is used widely in statistics to calculate the proportion of data values...
10.2K
01:19Enzyme Kinetics
104.2K
Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...
104.2K
01:24Data Reporting and Recording
5.5K
Reporting and recording are crucial in data documentation. The timely, thorough, and accurate documentation of facts is essential when recording patient data. Failure to record findings during an assessment or interpretation of a problem will result in loss of information and make the patient document unreliable. The reader is left with general impressions if the information is not specific. A recording is documenting data of the individual's health information in a traceable, secure, and...
5.5K