Rapid and persistent RNAi response in western corn rootworm adults.

Area of Science:

• Entomology
• Molecular Biology
• Pest Management

Background:

• RNA interference (RNAi) is a proven method for controlling insect pests like the western corn rootworm (WCR).
• WCR adults exhibit a strong RNAi response to ingested double-stranded RNA (dsRNA).
• The speed and duration of the RNAi response in WCR following dsRNA ingestion remain unclear.

Purpose of the Study:

• To determine the onset and duration of RNAi response in WCR adult females after dsRNA exposure.
• To assess the longevity of RNAi effects after WCR cease ingesting dsRNA-containing diets.

Main Methods:

• WCR adult females were fed a diet containing dsRNAs targeting Laccase 2 and Argonaute 2 for 8 days.
• RNAi response was monitored at various time points after initial exposure and after dsRNA withdrawal.

Main Results:

• RNAi response in WCR females was observed as early as 10 hours post-exposure to dsRNA.
• The RNAi effect persisted for up to 40 days after the WCR stopped ingesting the dsRNA-treated diet.
• The study demonstrates a rapid and sustained RNAi response in WCR females.

Conclusions:

• RNAi-mediated pest control in WCR is both rapid and long-lasting.
• Short-term ingestion of dsRNA, potentially from transgenic plants, can lead to sustained impacts on WCR populations.
• These findings support the potential of RNAi technology for effective and durable WCR management.