Related Experiment Video

Updated: Feb 5, 2026

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
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Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

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Gemcitabine-Incorporated G-Quadruplex Aptamer for Targeted Drug Delivery into Pancreas Cancer

Jun Young Park1, Ye Lim Cho2, Ju Ri Chae2

  • 1Department of Nuclear Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul, Korea; Department of Anatomy, Yonsei University Wonju College of Medicine, Wonju, Korea.

Molecular Therapy. Nucleic Acids
|September 10, 2018
PubMed

Insights

A novel aptamer-based gemcitabine delivery system, APTA-12, shows promise for pancreatic cancer treatment. This targeted therapy effectively inhibited cancer cell growth in vitro and in vivo, overcoming gemcitabine resistance.

Area of Science:

  • Oncology
  • Biotechnology
  • Drug Delivery

Background:

  • Gemcitabine is a first-line chemotherapy for pancreatic cancer but suffers from low response rates and frequent chemoresistance.
  • Aptamers offer targeted delivery of therapeutics into cancer cells due to their high affinity and specificity.

Purpose of the Study:

  • To construct and evaluate an aptamer-based gemcitabine delivery system (APTA-12) for pancreatic cancer therapy.
  • To assess the in vitro and in vivo therapeutic efficacy of APTA-12 in pancreatic cancer models.

Main Methods:

  • Construction of an aptamer-conjugated gemcitabine delivery system, APTA-12.
  • In vitro cytotoxicity assays on pancreatic cancer cell lines.
  • In vivo efficacy studies using Capan-1 tumor-bearing mice.

Main Results:

  • APTA-12 demonstrated significant cytotoxicity against pancreatic cancer cell lines in vitro.
  • In vivo studies showed APTA-12 effectively inhibited tumor growth in mice compared to gemcitabine alone.
  • Therapeutic effects were observed in pancreatic cancer cells with high nucleolin expression.

Conclusions:

  • The gemcitabine-incorporated APTA-12 aptamer system is a potential targeted therapeutic strategy for pancreatic cancer.
  • APTA-12 may overcome gemcitabine resistance and improve treatment outcomes for pancreatic cancer patients.
Keywords:
G-quadruplexaptameraptamer-drug conjugatesgemcitabinepancreatic cancer

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