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Ent-kaurane diterpenoids from the cherries of Coffea arabica
Xia Wang1, Xingrong Peng2, Jing Lu1
1State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, People's Republic of China; University of the Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
Abstract:
Four new rearranged ent-kaurane diterpenoids named as caffruenol A (1), caffruenol B (2), caffruolide A (3), caffruolide B (4), along with eleven known analogues (5-15) were isolated from the cherries of Coffea arabica. The structures of new compounds were elucidated by extensive spectroscopic analysis and X-ray crystallography. Coffruenols A and B (1 and 2) possessed an ent-4(18)-kaurene framework and might play an important role on the biosynthesis of the rearranged diterpenes that occurred in C. arabica. Moreover, inhibitory effects of compounds 1-4 on nitric oxide (NO) production in lipopolysaccharide-activited RAW 264.7 macrophages were evaluated.
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