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Updated: Feb 5, 2026

Isolation and Transplantation of Hematopoietic Stem Cells HSCs
Published on: February 25, 2007
Identification and Isolation of Mice and Human Hematopoietic Stem Cells
Bijender Kumar1, Srideshikan Sargur Madabushi2
1Department of Radiation Oncology, City of Hope Medical Center, Duarte, CA, USA. bkumar@coh.org.
Abstract:
Hematopoietic stem cells (HSCs) are multipotent cells capable of differentiating into all types of blood cells. The important feature of the HSCs is their ability to repopulate the complete blood cells after BM ablation. For clinical application, cord blood derived HSCs and G-CSF mobilized peripheral blood HSCs are good alternative to bone marrow HSCs. For immunological and hematological studies the obvious choice of model organism is Mouse. Therefore, understanding HSCs in murine model is important. In this chapter, we describe the common/currently used methods to isolate and identify human and mouse HSCs.
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