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Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
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Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
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Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
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Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
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Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
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A stem cell is an unspecialized cell that can divide without limit as needed and can, under specific conditions, differentiate into specialized cells.
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Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues
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Isolating and Characterizing Adipose-Derived Stem Cells.

Guangpeng Liu1, Xi Chen2

  • 1Department of Plastic and Reconstructive Surgery, Shanghai Tenth People's Hospital, TongJi University School of Medicine, Shanghai, People's Republic of China. guangpengliu@163.com.

Methods in Molecular Biology (Clifton, N.J.)
|September 10, 2018
PubMed
Summary

Adipose-derived stem cells (ASCs) offer a plentiful and accessible source for regenerative medicine. This study details methods for isolating and characterizing these valuable adult stem cells from adipose tissue for potential clinical use.

Keywords:
Adipose tissueAdult stem cellsCharacterizationIsolationMultilineage differentiation

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Area of Science:

  • Regenerative Medicine
  • Stem Cell Biology
  • Tissue Engineering

Background:

  • Regenerative medicine relies on a consistent supply of stem cells.
  • Adipose tissue is an abundant and easily accessible source of adult stem cells.
  • Adipose-derived stem cells (ASCs) present a unique advantage due to tissue abundance.

Purpose of the Study:

  • To describe detailed methodologies for the isolation of ASCs.
  • To outline characterization techniques for ASCs.
  • To provide adaptable procedures for human and other species' adipose tissue.

Main Methods:

  • Isolation of stem cells from adipose tissue.
  • Characterization of isolated adipose-derived stem cells.
  • Standardized protocols applicable across species.

Main Results:

  • Established protocols for ASC isolation and characterization.
  • Demonstrated the feasibility of using adipose tissue as a stem cell source.
  • Confirmed the abundance of ASCs for clinical applications.

Conclusions:

  • ASCs are a readily available and abundant stem cell source.
  • Detailed methods facilitate ASC isolation and characterization.
  • These protocols support the advancement of regenerative medicine and clinical applications.