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Display Technologies for Generation of Ig Single Variable Domains

Vladimir Bobkov1, Bas van der Woning2, Hans de Haard2

  • 1argenx BVBA, Zwijnaarde, Belgium. vbobkov@argenx.com.

Methods in Molecular Biology (Clifton, N.J.)
|September 10, 2018
PubMed
Summary

Camelid heavy-chain-only antibody fragments (VHH) are crucial for research and clinical applications. This protocol details constructing VHH immune libraries and using phage display for rapid discovery of antigen-specific antibody clones.

Keywords:
CamelidaeHeavy-chain-only antibodyImmune libraryNanobodyPhage displaySingle-domain antibodyVHHVirus-like particle

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