Related Experiment Video

Updated: Feb 5, 2026

Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity
12:31

Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity

Published on: May 1, 2018

14.8K

A Streamlined Approach for the Construction of Large Yeast Surface Display Fab Antibody Libraries

Simon Krah1,2, Julius Grzeschik1, Simon Rosowski1

  • 1Institute for Organic Chemistry and Biochemistry, Technische Universität Darmstadt, Darmstadt, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|September 10, 2018
PubMed

Keywords:
Antibody engineeringFab fragmentGolden Gate cloningHit discoveryLibrary generationProtein engineeringYeast surface display

More Related Videos

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries12:55

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Published on: January 17, 2015

19.2K
Protein Engineering by Yeast Surface Display05:49

Protein Engineering by Yeast Surface Display

Published on: November 29, 2024

3.6K

Related Concept Videos

Yeast Signaling01:28

Yeast Signaling

Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
17.3K
Streamlines, Streaklines, and Pathlines01:18

Streamlines, Streaklines, and Pathlines

A streamline represents the trajectory that is always tangent to the fluid's velocity vector at any given point. The velocity of a fluid particle is always directed along the streamline, ensuring the particle continuously follows the streamline's path. Streamlines are particularly useful for visualizing the overall direction of flow in a fluid system, and they provide an instantaneous representation of the flow's velocity field. In steady flow, where conditions do not change over...
2.0K
Bernoulli's Equation for Flow Along a Streamline01:30

Bernoulli's Equation for Flow Along a Streamline

Bernoulli's equation relates the energy conservation in a fluid moving along a streamline. The equation applies to incompressible and inviscid fluids under steady flow. For such a flow, Newton's second law is applied to a small fluid element, which experiences forces due to pressure differences, gravity, and velocity variations. The force balance leads to the following form of Bernoulli's equation:
1.5K
Antibody Structure01:10

Antibody Structure

Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
65.6K
Bernoulli's Equation for Flow Normal to a Streamline01:16

Bernoulli's Equation for Flow Normal to a Streamline

Bernoulli's equation for flow normal to a streamline explains how pressure varies across curved streamlines due to the outward centrifugal forces induced by the fluid's curvature. The pressure is higher on the inner side of the curve, near the center of curvature, and decreases outward to balance these centrifugal forces.
The pressure difference depends on the fluid's velocity and radius of curvature. The pressure variation is minimal in flows with nearly straight streamlines. However, the...
1.3K
Surface Tension and Surface Energy01:16

Surface Tension and Surface Energy

When a paint brush is immersed in water, the bristles wave freely inside the water. When it is taken out, the bristles stick together. The reason behind this effect is surface tension.
Consider a beaker filled with liquid. The bulk molecules in the liquid experience equal attractive forces on all sides with the surrounding molecules. However, the surface molecules experience a net attractive force downward due to the bulk molecules. The surface of the liquid behaves like a stretched membrane,...
3.3K