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Related Concept Videos

Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

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Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
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Induced Pluripotent Stem Cells01:06

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Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
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Embryonic Stem Cells00:58

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Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
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Embryonic Stem Cells00:57

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Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
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Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
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Rapid and Efficient Generation of Recombinant Human Pluripotent Stem Cells by Recombinase-mediated Cassette Exchange in the AAVS1 Locus
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Rapid Mast Cell Generation from Gata2 Reporter Pluripotent Stem Cells.

Mari-Liis Kauts1, Bianca De Leo2, Carmen Rodríguez-Seoane2

  • 1Erasmus Stem Cell Institute, Department of Cell Biology, Erasmus Medical Center, Rotterdam, Netherlands; MRC Centre for Inflammation Research, Queen's Medical Research Institute, University of Edinburgh, 47 Little France Crescent, Edinburgh EH16 4TJ, UK.

Stem Cell Reports
|September 11, 2018
PubMed
Summary

Researchers developed a rapid method to generate mast cells from pluripotent stem cells (PSCs). This approach overcomes limitations of current methods, enabling faster research into mast cell disorders and drug discovery.

Keywords:
ESCGata2Venus reporterdifferentiationiPSCimmune effectorsinnate immune cellsmast cellsrapid protocolstem cells

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Area of Science:

  • Immunology
  • Stem Cell Biology
  • Drug Discovery

Background:

  • Mast cells are crucial immune cells implicated in various disorders like asthma and anaphylaxis.
  • Current methods for mast cell generation are inefficient, time-consuming, and lack reproducibility.
  • Existing mast cell lines and primary cell cultures do not fully represent in vivo conditions.

Purpose of the Study:

  • To develop a rapid and robust method for mast cell production from pluripotent stem cells (PSCs).
  • To overcome the limitations of current mast cell generation techniques for research and drug discovery.

Main Methods:

  • Utilized pluripotent stem cells (PSCs) for mast cell generation.
  • Employed a Gata2Venus reporter system to enrich mast cells and progenitors during differentiation.
  • Cultured cells for a 2-week period to achieve rapid proliferation.

Main Results:

  • Successfully generated highly proliferative mouse mast cells and progenitors within 2 weeks.
  • Demonstrated the applicability of the method for rapid human mast cell generation.
  • The Gata2Venus reporter facilitated efficient enrichment of mast cells and progenitors.

Conclusions:

  • The novel PSC-based method provides a rapid and robust way to produce physiologically relevant mast cells.
  • This advancement can significantly aid in studying mast cell-associated disorders and accelerate drug discovery.
  • Enables the generation of sufficient mast cells from patient-derived iPSCs for personalized medicine approaches.