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Seong-Cheol Moon1,2, Hee-Jung Choi1,2, Tae-Wook Chung1,2

  • 1Department of Korean Medical Science, School of Korean Medicine, Pusan National University, Yangsan, Gyeongnam 50612, Republic of Korea.

Oncology Letters
|September 11, 2018
PubMed
Summary

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The water extract of Sorbus commixta stembark (SCE) shows potent anticancer effects by inducing apoptosis in liver and colon cancer cells. Its mechanism involves the reactive oxygen species (ROS)-mediated mitochondrial pathway.

Area of Science:

  • Phytochemistry
  • Molecular Biology
  • Cancer Research

Background:

  • The stembark of Sorbus commixta Hedl. is traditionally used for various ailments.
  • The anticancer and proapoptotic potential of Sorbus commixta extract (SCE) is not well-established.

Purpose of the Study:

  • To investigate the anticancer and proapoptotic effects of SCE.
  • To elucidate the underlying molecular mechanisms of SCE's action.

Main Methods:

  • Cell viability assays on hepatocellular carcinoma (Hep3B, HepG2) and colon carcinoma (HCT116) cell lines.
  • DNA content analysis, caspase activation assays, and mitochondrial membrane potential assessment.
  • Western blotting for apoptosis-related proteins (p53, p21, p27, Bcl-2, Bax, MDM2) and reactive oxygen species (ROS) measurement.
Keywords:
Sorbus commixtaapoptosismitochondriareactive oxygen species

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Main Results:

  • SCE significantly inhibited cell viability in tested cancer cell lines.
  • SCE induced DNA fragmentation and apoptosis, evidenced by increased sub-G1 population and caspase activation.
  • SCE modulated mitochondrial pathways by reducing membrane potential and altering Bcl-2/Bax ratio, and increased ROS production.
  • SCE upregulated proapoptotic proteins (p53, p21, p27) and cleaved MDM2.
  • N-acetyl cysteine (ROS inhibitor) attenuated SCE's cytotoxic effects and proapoptotic markers.

Conclusions:

  • SCE exhibits significant anticancer properties by inducing apoptosis in cancer cells.
  • The proapoptotic effects of SCE are mediated through the ROS-dependent mitochondrial pathway.
  • SCE represents a potential herbal therapeutic agent for cancer treatment.