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Detection of Plasmid-Mediated

Reza Ranjbar1, Sajjad S Tolon1, Mehrdad Sami2

  • 1Molecular Biology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.

The Open Microbiology Journal
|September 11, 2018
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Summary

Multidrug-resistant Escherichia coli strains causing urinary tract infections (UTIs) are a global health concern. This study found the qnrS gene in 92% of nalidixic acid-resistant E. coli from Iranian patients, highlighting the need for further research.

Keywords:
Antibiotic resistanceClinical strainsE. coliPCRUTIsqnr

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Area of Science:

  • Microbiology
  • Infectious Diseases
  • Genetics

Background:

  • Escherichia coli is a primary cause of urinary tract infections (UTIs).
  • Rising antibiotic resistance in E. coli, particularly multidrug resistance, limits effective treatment options.
  • The global increase in resistant bacterial strains is a significant public health concern.

Purpose of the Study:

  • To investigate the prevalence of qnr genes (qnrA, qnrB, qnrS) in E. coli isolates from patients with UTIs.
  • To analyze the genetic basis of quinolone resistance in E. coli from a specific Iranian hospital.

Main Methods:

  • Collected clinical urine samples from patients with suspected UTIs.
  • Extracted bacterial DNA using a boiling method.
  • Employed Polymerase Chain Reaction (PCR) with specific primers to detect qnrA, qnrB, and qnrS genes.
  • Utilized gel electrophoresis and gel documentation for PCR product visualization.

Main Results:

  • Out of 95 E. coli isolates, 60 strains exhibited resistance to nalidixic acid.
  • PCR analysis revealed the presence of the qnrS gene in 92 of the isolates.
  • The qnrA and qnrB genes were not detected in any of the clinical isolates.

Conclusions:

  • A high prevalence of nalidixic acid resistance was observed in E. coli isolates from UTI patients.
  • The significant frequency of the qnrS gene underscores the importance of molecular and genetic studies to understand quinolone resistance mechanisms in E. coli.