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lncRNA - Long Non-coding RNAs02:39

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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Updated: Feb 5, 2026

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
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Knockdown of long non-coding RNA

Xiao Yu1, Xiaofei Ye1, Hongyan Lin1

  • 1Hongqiao International Institute of Medicine, Shanghai Tongren Hospital and Faculty of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.

Cancer Biology & Medicine
|September 11, 2018
PubMed
Summary
This summary is machine-generated.

Lung cancer progression-associated transcript 1 (LCPAT1) regulates autophagy. Inhibiting LCPAT1 suppressed lung cancer growth and cell autophagy, suggesting LCPAT1 is crucial for lung cancer progression.

Keywords:
ENST00000439577LCAPT1Lung cancerautophagylong non-coding RNA

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Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • Long non-coding RNAs (lncRNAs) play roles in lung cancer.
  • A novel lncRNA, LCPAT1, is highly expressed in lung carcinomas.

Purpose of the Study:

  • To investigate the role of LCPAT1 in lung cancer progression.
  • To determine the relationship between LCPAT1 and autophagy in lung cancer cells.

Main Methods:

  • Real-time qPCR to measure LCPAT1 and autophagy marker expression.
  • In vivo xenograft models and in vitro cell lines were used.
  • Western blot, immunofluorescence, and HE staining evaluated tumor tissues and autophagy.

Main Results:

  • LCPAT1 expression positively correlated with LC3B, an autophagy marker.
  • LCPAT1 knockdown inhibited tumor growth and suppressed autophagy in vivo.
  • LCPAT1 knockdown decreased autophagy-associated gene expression and rapamycin-induced autophagy.

Conclusions:

  • LCPAT1 plays a significant role in regulating autophagy in lung cancer.
  • LCPAT1 may be a potential therapeutic target for lung cancer treatment.