Data on degradome sequencing and analysis from mock-inoculated and

Area of Science:

• Molecular Biology
• Plant Science
• Genomics

Background:

• Degradome sequencing, a modification of 5'-rapid amplification of cDNA ends (RACE) coupled with deep sequencing, enables large-scale determination of RNA cleavage sites.
• Small RNAs, including microRNAs (miRNAs) and small interfering RNAs (siRNAs), play crucial roles in post-transcriptional gene regulation.

Purpose of the Study:

• To identify small RNA (sRNA) cleavage sites in the medicinal plant Persicaria minor.
• To investigate the impact of Fusarium oxysporum infection on sRNA targeting in P. minor.

Main Methods:

• Degradome sequencing (Parallel Analysis of RNA Ends - PARE) was performed on pooled samples from control (mock-inoculated) and F. oxysporum-treated P. minor plants.
• Deep sequencing of 5' cDNA products allowed for the mapping of degradome fragments against known sRNAs to pinpoint cleavage sites.

Main Results:

• The study successfully generated a degradome dataset (D4) from P. minor, encompassing both control and treated conditions.
• Analysis of the D4 dataset provided insights into sRNA-mediated cleavage events within the plant, particularly in response to F. oxysporum.

Conclusions:

• Degradome sequencing is an effective method for identifying sRNA targets in plants like P. minor.
• The findings contribute to understanding plant-pathogen interactions at the molecular level through sRNA-mediated gene regulation.