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Proteomic and functional data sets on synaptic mitochondria from rats with genetic ablation of
Lance M Villeneuve1, Kelly L Stauch1, Phillip S Purnell1
1Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE 68198, United States.
Data in Brief
|September 11, 2018
Summary
Parkin ablation in rat striatum affects synaptic mitochondria. This study provides proteomic and functional data on these mitochondria, aiding Parkinson's disease research.
Area of Science:
- Neuroscience
- Mitochondrial Biology
- Proteomics
Background:
- Parkinson's disease is linked to mitochondrial dysfunction.
- Parkin is a key protein involved in mitochondrial quality control.
- Synaptic mitochondria are crucial for neuronal function.
Purpose of the Study:
- To characterize synaptic mitochondria from Parkin-ablated rat striatum.
- To provide quantitative proteomic and functional data.
- To enable comparison with existing datasets.
Main Methods:
- Quantitative proteomics using SWATH-MS methodology.
- Mitochondrial function assessment via oxygen consumption rate measurements.
- Utilizing the Seahorse XF Analyzer for functional assays.
Main Results:
- Detailed proteomic profiles of synaptic mitochondria from Parkin-ablated rats.
- Functional data on mitochondrial respiration in the absence of Parkin.
- Comprehensive dataset for comparative analysis.
Conclusions:
- The study provides valuable data on mitochondrial changes in a model of Parkin deficiency.
- This resource facilitates further investigation into the role of Parkin in mitochondrial health.
- The findings contribute to understanding the molecular basis of Parkinson's disease.
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