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Identification of Circular RNAs using RNA Sequencing
08:25

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

12.8K

The Circular RNA

Malte Kölling1, Harald Seeger1, George Haddad1

  • 1Division of Nephrology, University Hospital Zürich, Zürich, Switzerland.

Kidney International Reports
|September 11, 2018
PubMed
Abstract

Insights

Circulating circular RNAs (circRNAs) are detectable in critically ill patients with acute kidney injury (AKI). A specific circRNA, ciRs-126, may predict mortality in AKI patients.

Area of Science:

  • Molecular Biology
  • Genomics
  • Biochemistry

Background:

  • Circular RNAs (circRNAs) are novel noncoding RNA regulators with potential roles in microRNA (miRNA) expression.
  • circRNAs are detectable in blood and have been implicated in cancer and cardiovascular diseases.
  • The presence and role of circRNAs in acute kidney injury (AKI) remain largely unexplored.

Purpose of the Study:

  • To investigate the dysregulation and potential role of circulating circRNAs in critically ill patients with AKI.
  • To determine if circulating circRNAs are associated with patient survival in AKI.

Main Methods:

  • Global circRNA expression analysis was performed on blood samples from AKI patients and controls.
  • Quantitative real-time polymerase chain reaction (qRT-PCR) was used to validate key circRNAs and their targets in a larger cohort.
  • Statistical analyses, including Cox regression and Kaplan-Meier curves, were employed to assess survival prediction.

Main Results:

  • Three novel circRNAs were found to be upregulated in the blood of AKI patients.
  • Circular RNA sponge of miR-126 (ciRs-126) showed a significant 52.1-fold increase in AKI patients compared to controls.
  • ciRs-126 was identified as a potential sponge for miR-126-5p, which was suppressed in AKI patients and hypoxic cells.

Conclusions:

  • Circulating circRNAs are detectable in patients with AKI.
  • ciRs-126 may function as a miRNA sponge for miR-126-5p.
  • ciRs-126 is an independent predictor of 28-day mortality in AKI patients.
Keywords:
acute kidney injurycirculating circular RNAsmortalityrenal replacement therapy

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