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Sydnone-coumarins as clickable turn-on fluorescent sensors for molecular imaging
Elodie Decuypère1, Margaux Riomet, Antoine Sallustrau
1Service de Chimie Bio-organique et Marquage DRF-JOLIOT-SCBM, CEA, Université Paris-Saclay, 91191 Gif-sur-Yvette, France. frederic.taran@cea.fr.
Abstract:
Copper-catalyzed and copper-free sydnone-alkyne cycloaddition reactions have emerged as complementary click tools for chemical biology but their use in bioorthogonal labeling is still in its infancy. Herein, combinations of alkynes and coumarin-sydnones were screened for their ability to generate pyrazole products displaying strong fluoroscence enhancement compared to reactants. One sydnone was identified as a particularly suitable new turn-on probe for protein labeling.
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