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Agarose-based Tissue Mimicking Optical Phantoms for Diffuse Reflectance Spectroscopy
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Agarose-based Tissue Mimicking Optical Phantoms for Diffuse Reflectance Spectroscopy.

Afrina Mustari1, Izumi Nishidate2, Md Abdul Wares3

  • 1Graduate School of Bio-application & Systems Engineering, Tokyo University of Agriculture & Technology.

Journal of Visualized Experiments : Jove
|September 11, 2018
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Summary

This study presents a protocol for creating tissue-mimicking phantoms using agarose. Researchers can determine optical properties, like absorption and scattering, of these phantoms for biomedical applications.

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Area of Science:

  • Biomedical Optics
  • Tissue Phantoms
  • Optical Property Measurement

Background:

  • Accurate optical properties of tissue phantoms are crucial for developing optical imaging and sensing technologies.
  • Conventional methods for phantom characterization can be complex and time-consuming.

Purpose of the Study:

  • To describe a protocol for fabricating agarose-based tissue-mimicking phantoms.
  • To demonstrate a method for determining the optical properties of these phantoms using a conventional optical system.

Main Methods:

  • Fabrication of epidermal and dermal phantoms using acrylic molds.
  • Measurement of diffuse reflectance and total transmittance spectra using an integrating sphere system.
  • Application of inverse Monte Carlo simulation to extract absorption and reduced scattering coefficients.

Main Results:

  • Successful creation of single-layer phantoms (epidermal and dermal) with tunable optical properties.
  • Demonstration of a two-layered phantom mimicking human skin's diffuse reflectance.
  • Quantification of absorption coefficient spectrum (µa(λ)) and reduced scattering coefficient spectrum (µs'(λ)).

Conclusions:

  • The described protocol provides a reliable method for creating and characterizing tissue-mimicking phantoms.
  • These phantoms serve as valuable tools for validating optical techniques in biomedical research.
  • The developed system enables precise determination of optical properties essential for various applications.