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Routine Screening Method for Microparticles in Platelet Transfusions
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Analytical validation of platelet microparticle quantification in cats.

Signe E Cremer1, Jørgen Koch1, Nanna Graversen1

  • 1Department of Veterinary Clinical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.

Veterinary Clinical Pathology
|September 11, 2018
PubMed
Summary

Platelet microparticles (PMPs) can be reliably measured in cat whole blood, but concentrations did not differ between healthy cats and those with asymptomatic hypertrophic cardiomyopathy (HCM) in this pilot study.

Keywords:
AnnexinCD61cardiacmicrovesiclesprecision

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Area of Science:

  • Veterinary Medicine
  • Cardiology
  • Hematology

Background:

  • Cardiogenic embolism (CE) in cats is a serious condition often linked to hypertrophic cardiomyopathy (HCM).
  • Hypercoagulability is a risk factor for thrombus formation, but predicting CE is challenging.
  • Platelet microparticles (PMPs) are implicated in thrombosis in humans.

Purpose of the Study:

  • To analytically validate flow cytometric quantification of PMPs in cats.
  • To assess procoagulant annexin V (AnV) positive PMP concentrations in healthy cats and cats with asymptomatic HCM.

Main Methods:

  • Quantified CD61+ AnV+ PMPs (0.3-1.0 μm) using flow cytometry in whole blood (WB) and platelet-poor plasma (PPP).
  • Analyzed samples from 6 healthy cats and 5 cats with asymptomatic HCM.
  • Calculated intra-assay and inter-assay coefficients of variation (CV).

Main Results:

  • Whole blood PMP quantification showed acceptable intra-assay CV for both CD61+ /AnV- and CD61+ /AnV+ PMPs.
  • Inter-assay CV was acceptable for WB PMPs but unacceptable for PPP PMPs.
  • No significant difference in WB PMP concentrations (CD61+ /AnV- or CD61+ /AnV+) was observed between healthy cats and cats with asymptomatic HCM.

Conclusions:

  • Whole blood PMP quantification is reliable for cats in a clinical setting.
  • This pilot study found no difference in PMP concentrations between healthy cats and those with asymptomatic HCM.