Related Experiment Video

Updated: Feb 5, 2026

Routine Screening Method for Microparticles in Platelet Transfusions
09:49

Routine Screening Method for Microparticles in Platelet Transfusions

Published on: January 31, 2018

16.6K

Analytical validation of platelet microparticle quantification in cats

Signe E Cremer1, Jørgen Koch1, Nanna Graversen1

  • 1Department of Veterinary Clinical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.

Veterinary Clinical Pathology
|September 11, 2018
PubMed

Insights

Platelet microparticles (PMPs) can be reliably measured in cat whole blood, but concentrations did not differ between healthy cats and those with asymptomatic hypertrophic cardiomyopathy (HCM) in this pilot study.

Area of Science:

  • Veterinary Medicine
  • Cardiology
  • Hematology

Background:

  • Cardiogenic embolism (CE) in cats is a serious condition often linked to hypertrophic cardiomyopathy (HCM).
  • Hypercoagulability is a risk factor for thrombus formation, but predicting CE is challenging.
  • Platelet microparticles (PMPs) are implicated in thrombosis in humans.

Purpose of the Study:

  • To analytically validate flow cytometric quantification of PMPs in cats.
  • To assess procoagulant annexin V (AnV) positive PMP concentrations in healthy cats and cats with asymptomatic HCM.

Main Methods:

  • Quantified CD61+ AnV+ PMPs (0.3-1.0 μm) using flow cytometry in whole blood (WB) and platelet-poor plasma (PPP).
  • Analyzed samples from 6 healthy cats and 5 cats with asymptomatic HCM.
  • Calculated intra-assay and inter-assay coefficients of variation (CV).

Main Results:

  • Whole blood PMP quantification showed acceptable intra-assay CV for both CD61+ /AnV- and CD61+ /AnV+ PMPs.
  • Inter-assay CV was acceptable for WB PMPs but unacceptable for PPP PMPs.
  • No significant difference in WB PMP concentrations (CD61+ /AnV- or CD61+ /AnV+) was observed between healthy cats and cats with asymptomatic HCM.

Conclusions:

  • Whole blood PMP quantification is reliable for cats in a clinical setting.
  • This pilot study found no difference in PMP concentrations between healthy cats and those with asymptomatic HCM.
Abstract

Keywords:
AnnexinCD61cardiacmicrovesiclesprecision

More Related Videos

Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets05:49

Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets

Published on: November 29, 2024

1.2K
Functional Imaging of Auditory Cortex in Adult Cats using High-field fMRI10:50

Functional Imaging of Auditory Cortex in Adult Cats using High-field fMRI

Published on: February 19, 2014

12.0K

Related Concept Videos

Reliability and Validity01:29

Reliability and Validity

Reliability and validity are two important considerations that must be made with any type of data collection. Reliability refers to the ability to consistently produce a given result. In the context of psychological research, this would mean that any instruments or tools used to collect data do so in consistent, reproducible ways.
14.0K
Development of Analytical Methods01:21

Development of Analytical Methods

An analytical methodology can be divided into four sequential steps: technique, method, procedure, and protocol. A technique is a scientific principle that rationalizes a specific phenomenon through chemical measurements. Adapting a technique for analyzing a sample of interest is termed a method. The procedure outlines the directions for performing the analysis via an analytical method. The protocol is the detailed guidelines on the procedure, which should be strictly followed to obtain the...
2.3K
Analyte Adsorption and Distribution01:09

Analyte Adsorption and Distribution

In certain chromatographic separations, solutes transfer between the mobile phase and the stationary phase via sorption, which typically refers to the process of adsorption. For many chromatographic systems, the sorption process often depends on the polarity of the compounds—an expression of the overall dipole moment within the molecule. During the separation process, there is competition between the solute and solvent for adsorption to the stationary phase. Highly polar compounds and...
2.8K
Jung's Analytical Theory01:23

Jung's Analytical Theory

Carl Jung, a Swiss psychiatrist and former follower of Freud, eventually broke away from Freud's ideas to create his framework, analytical psychology. This approach emphasizes achieving a balance between the conscious and unconscious aspects of the mind and reconciling various experiences within an individual's personality. Jung believed that this process, which typically unfolds in the latter part of life, involves an ongoing journey of recognizing and incorporating unconscious...
1.1K
Data Validation01:15

Data Validation

Method validation is a crucial process in analytical chemistry designed to confirm that a given method consistently produces reliable and high-quality results. This process is essential when a method is applied to different sample matrices or when procedural modifications are made, ensuring that the results meet acceptable standards across various applications.
Key parameters for method validation include:
2.0K
Data Validation01:03

Data Validation

Data validation is an essential part of a comprehensive assessment. Validation is confirming or verifying and opening the door to gathering more assessment data as it clarifies vague or unclear data. The process of checking and verifying the collected information is called data validation. The primary purpose of data validation is to ensure data is as free from error, bias, and misinterpretation as possible.
Nursing assessment guides are generally based on holistic models rather than medical...
6.8K