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Stability control for breath analysis using GC-MS.

X Rosalind Wang1, Julie Cassells2, Amalia Z Berna3

  • 1CSIRO Data61, PO Box 76, Epping, NSW 1710, Australia.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
|September 11, 2018
PubMed
Summary
This summary is machine-generated.

Gas chromatography mass spectrometry (GC-MS) instruments are crucial for breath analysis but susceptible to data variability. This study reveals instrument drift and biomarker degradation, recommending data normalization and cold storage for reliable results.

Keywords:
BreathDay of analysisGas chromatographyMass spectrometryStabilityThioethersVariability

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Area of Science:

  • Analytical Chemistry
  • Biomarker Discovery

Background:

  • Gas chromatography mass spectrometry (GC-MS) is the gold standard for breath analysis.
  • Data interpretation in breath analysis research can be confounded by various factors.
  • Instrument variation and chemical degradation impact GC-MS data reliability.

Purpose of the Study:

  • To investigate instrument variation over time in GC-MS.
  • To assess the chemical degradation of biomarkers during storage in sorbent tubes.
  • To provide recommendations for improving data reliability in breath analysis.

Main Methods:

  • Comparative analysis of single quadrupole MS and quadrupole time-of-flight MS.
  • Stability studies of biomarkers, specifically thioethers, in sorbent tubes.
  • Longitudinal analysis of chemical standard mixtures over eight weeks.

Main Results:

  • Single quadrupole MS exhibited greater measurement variability compared to quadrupole time-of-flight MS.
  • Thioethers, potential malaria biomarkers, showed significant degradation when stored at room temperature.
  • Optimal storage conditions identified as 6°C for no more than 20 days to preserve biomarker integrity.

Conclusions:

  • Implementing data normalization procedures is recommended to mitigate instrument variation.
  • Low-temperature storage (6°C) is critical for preserving biomarker stability in sorbent tubes.
  • Adherence to recommended storage durations and temperatures is essential for accurate breath analysis research.