Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Atomic Absorption Spectroscopy: Radiation and Light Sources01:13

Atomic Absorption Spectroscopy: Radiation and Light Sources

1.3K
Atomic absorption spectroscopy (AAS) relies on the Beer-Lambert law, which requires that the radiation source emits a narrow range of wavelengths to match the absorption characteristics of the analyte atom. The primary criteria for choosing an appropriate radiation source in AAS is to provide a precise and intense emission at specific wavelengths that will allow accurate detection of the analyte.
Two common narrow-range 'line' sources used in AAS are hollow-cathode lamps (HCLs) and...
1.3K
The Sense of Self: Reflected Self-Appraisal and Social Comparison02:57

The Sense of Self: Reflected Self-Appraisal and Social Comparison

56.1K
According to Charles Cooley, we base our image on what we think other people see (Cooley 1902). We imagine how we must appear to others, then react to this speculation. We don certain clothes, prepare our hair in a particular manner, wear makeup, use cologne, and the like—all with the notion that our presentation of ourselves is going to affect how others perceive us. We expect a certain reaction, and, if lucky, we get the one we desire and feel good about it. But more than that, Cooley...
56.1K
Multiple Comparison Tests01:13

Multiple Comparison Tests

4.5K
Multiple comparison test, abbreviated as MCT, is a post hoc analysis generally performed after comparing multiple samples with one or more tests. An MCT will help identify a significantly different sample among multiple samples or a factor among multiple factors.
It would be easy to compare two samples using a significance alpha level of 0.05. In other words, there is only one sample pair to be compared. However, it would be difficult to identify a significantly different sample if the number...
4.5K
Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

7.0K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
7.0K
Light Acquisition02:16

Light Acquisition

9.6K
In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.
9.6K
Light as Energy01:35

Light as Energy

96.0K
The energy required to carry out photosynthesis is light— typically electromagnetic radiation from the sun. The range of all possible wavelengths is known as the electromagnetic spectrum.
Photons
A photon is a discrete electromagnetic particle or bundle of energy. Photons are characterized by their frequency, wavelength, and amplitude, similar to the properties of a wave. Waves with higher frequencies transmit more energy and have shorter wavelengths than longer wavelengths that transmit...
96.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Predicting longevity-related traits in Swiss low-input and organic dairy cows from herdbook data of first versus second lactation.

Veterinary and animal science·2026
Same author

Effects of production, fertility, health, management and conformation scores on longevity in six Swiss dairy breeds.

Veterinary and animal science·2026
Same author

Unraveling Synthetase's Mode of Action: The Pyrrolysyl-tRNA Synthetase Dimer Uses Secondary Binding Sites in the Cell.

Angewandte Chemie (International ed. in English)·2026
Same author

Heisenberg spin exchange and relaxation dynamics in EPR oximetry: A photoinduced transition from non-adiabatic rapid-sweeps to the rapid-scan regime.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same author

Cysteine-Targeting Gd-Based Spin Label and Its Application in Electron Paramagnetic Resonance Spectroscopy.

Bioconjugate chemistry·2025
Same author

Economic drivers of the optimal productive lifespan of dairy cows in two different Swiss milk production systems.

Journal of dairy science·2025

Related Experiment Video

Updated: Feb 5, 2026

Quantitative Analysis of Vacuum Induction Melting by Laser-induced Breakdown Spectroscopy
03:49

Quantitative Analysis of Vacuum Induction Melting by Laser-induced Breakdown Spectroscopy

Published on: June 10, 2019

7.7K

Light-induced dipolar spectroscopy - A quantitative comparison between LiDEER and LaserIMD.

Anna Bieber1, Dennis Bücker1, Malte Drescher1

  • 1Department of Chemistry and Konstanz Research School Chemical Biology, University of Konstanz, Universitätsstraße 10, 78464 Konstanz, Germany.

Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|September 11, 2018
PubMed
Summary

Comparing two light-induced electron paramagnetic resonance (EPR) spectroscopy techniques for nanometric distance measurements, this study found chirp-enhanced LiDEER improves performance. Chirp LiDEER and LaserIMD show technique-dependent signal-to-noise ratios based on dipolar evolution time.

Keywords:
AWGChirp pulsesDEEREPRLaserIMDLiDEERLight-induced PELDORLight-induced dipolar spectroscopyPELDORPorphyrinsPulsed EPRTriplet state

More Related Videos

Diffuse Optical Spectroscopy for the Quantitative Assessment of Acute Ionizing Radiation Induced Skin Toxicity Using a Mouse Model
06:21

Diffuse Optical Spectroscopy for the Quantitative Assessment of Acute Ionizing Radiation Induced Skin Toxicity Using a Mouse Model

Published on: May 27, 2016

8.6K
Quantitative Comparison of cis-Regulatory Element CRE Activities in Transgenic Drosophila melanogaster
08:19

Quantitative Comparison of cis-Regulatory Element CRE Activities in Transgenic Drosophila melanogaster

Published on: December 19, 2011

12.3K

Related Experiment Videos

Last Updated: Feb 5, 2026

Quantitative Analysis of Vacuum Induction Melting by Laser-induced Breakdown Spectroscopy
03:49

Quantitative Analysis of Vacuum Induction Melting by Laser-induced Breakdown Spectroscopy

Published on: June 10, 2019

7.7K
Diffuse Optical Spectroscopy for the Quantitative Assessment of Acute Ionizing Radiation Induced Skin Toxicity Using a Mouse Model
06:21

Diffuse Optical Spectroscopy for the Quantitative Assessment of Acute Ionizing Radiation Induced Skin Toxicity Using a Mouse Model

Published on: May 27, 2016

8.6K
Quantitative Comparison of cis-Regulatory Element CRE Activities in Transgenic Drosophila melanogaster
08:19

Quantitative Comparison of cis-Regulatory Element CRE Activities in Transgenic Drosophila melanogaster

Published on: December 19, 2011

12.3K

Area of Science:

  • Biophysical Chemistry
  • Spectroscopy
  • Macromolecular Structure

Background:

  • Electron paramagnetic resonance (EPR) spectroscopy enables nanometric distance measurements, crucial for understanding macromolecular structure and interactions.
  • Porphyrin triplet states offer a new class of light-inducible spin labels, expanding the scope of EPR distance measurements.
  • Two techniques, light-induced triplet-nitroxide DEER (LiDEER) and laser-induced magnetic dipole spectroscopy (LaserIMD), have been used for triplet-nitroxide distance measurements.

Purpose of the Study:

  • To quantitatively compare LiDEER and LaserIMD techniques under identical Q-band conditions.
  • To evaluate the effectiveness of chirp inversion pulses in improving LiDEER performance.
  • To determine the optimal technique based on dipolar evolution time for enhanced signal-to-noise ratio.

Main Methods:

  • Quantitative comparison of LiDEER and LaserIMD at Q-band.
  • Implementation of LiDEER with both rectangular and chirp inversion pump pulses.
  • Measurement of modulation depth and modulation-to-noise ratio at different dipolar evolution times (τ=2μs and τ=6μs).

Main Results:

  • Standard LiDEER with rectangular pulses showed lower modulation depth compared to LaserIMD.
  • Employing chirp inversion pulses in LiDEER significantly improved modulation depth.
  • LaserIMD provided higher modulation-to-noise ratios at short evolution times (τ=2μs), while chirp LiDEER excelled at longer times (τ=6μs) due to triplet spin phase memory.

Conclusions:

  • Chirp-enhanced LiDEER is a viable and improved method for triplet-nitroxide distance measurements.
  • The choice between chirp LiDEER and LaserIMD depends on the specific dipolar evolution time required for the experiment.
  • These findings advance the application of light-induced EPR spectroscopy for precise macromolecular structural analysis.