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Summary
This summary is machine-generated.

Panax notoginseng stem and leaf flavonoids were extracted using optimized methods. These extracts show significant antioxidant and skin-whitening properties, suggesting potential for beauty products.

Keywords:
Panax notoginsengantioxidantflavonoidsmouse melanoma B16 cellssurfactant

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Area of Science:

  • Agricultural Science
  • Biotechnology
  • Cosmetic Science

Background:

  • Panax notoginseng (P. notoginseng) stem and leaf are rich in flavonoids but often discarded as agricultural waste.
  • Limited research exists on P. notoginseng stem leaf flavonoid extraction and functional applications.
  • This study addresses the underutilization of P. notoginseng agricultural byproducts.

Purpose of the Study:

  • To optimize the extraction process for Panax notoginseng stem leaf flavonoids (SLPF).
  • To evaluate the functional properties of SLPF extracts, focusing on antioxidant and skin-whitening potential.
  • To explore the development of P. notoginseng stem leaf as a source for cosmetic ingredients.

Main Methods:

  • Response Surface Methodology (RSM) with Box-Behnken Design (BBD) was employed to optimize SLPF extraction.
  • Key extraction parameters optimized include time, ethanol concentration, SDS content, and liquid-to-material ratio.
  • Antioxidant activities (reducing, DPPH, superoxide anion, ABTS) and anti-melanin deposition effects on B16 cells were assessed.

Main Results:

  • Optimal extraction conditions yielded 2.10% total SLPF.
  • The extracts demonstrated significant antioxidant capacities with low EC50 values for various assays.
  • Effective inhibition of tyrosinase and melanin synthesis was observed, with IC50 values of 0.045 and 0.046 mg/mL, respectively.

Conclusions:

  • The optimized extraction process enhances the utilization rate and reduces costs associated with P. notoginseng stem leaf.
  • P. notoginseng stem leaf extracts possess potent antioxidant and skin-whitening properties.
  • These findings support the potential development of P. notoginseng stem leaf extracts for use in food and cosmetic products.