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Related Experiment Video

Updated: Feb 5, 2026

Detection of Enterohemorrhagic Escherichia Coli Colonization in Murine Host by Non-invasive In Vivo Bioluminescence System
06:20

Detection of Enterohemorrhagic Escherichia Coli Colonization in Murine Host by Non-invasive In Vivo Bioluminescence System

Published on: April 9, 2018

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Enterohemorrhagic

C Ming Tse1, Julie G In2,3, Jianyi Yin4

  • 1Department of Medicine, Division of Gastroenterology & Hepatology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. mtse@jhmi.edu.

Toxins
|September 12, 2018
PubMed

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Summary
This summary is machine-generated.

Extracellular serine protease P (EspP) from Shiga-toxin-producing Escherichia coli stimulates ion transport in human colonoids, contributing to watery diarrhea. This EspP-induced effect is independent of protease activity and CFTR, but partially dependent on calcium.

Area of Science:

  • Microbiology
  • Gastroenterology
  • Pathophysiology

Background:

  • Diarrheagenic Escherichia coli (E. coli) cause watery diarrhea, but the exact mechanisms remain unclear.
  • Shiga toxin and type-3 secretion system components do not independently induce fluid secretion.
  • Extracellular serine protease P (EspP) is secreted by diarrheagenic E. coli and its role as an enterotoxin is investigated.

Purpose of the Study:

  • To determine if EHEC-secreted EspP acts as an enterotoxin by stimulating ion transport in human colonoid monolayers (HCM).
  • To investigate the mechanisms underlying EspP-induced ion transport, including protease activity, CFTR mediation, and calcium dependence.

Main Methods:

  • Utilized adult stem-cell-derived human colonoid monolayers (HCM) as a model system.
Keywords:
CFTREHECSPATEsdiarrheahuman colonoid monolayersintracellular Ca2+serine protease EspPshort circuit current

Related Experiment Videos

Last Updated: Feb 5, 2026

Detection of Enterohemorrhagic Escherichia Coli Colonization in Murine Host by Non-invasive In Vivo Bioluminescence System
06:20

Detection of Enterohemorrhagic Escherichia Coli Colonization in Murine Host by Non-invasive In Vivo Bioluminescence System

Published on: April 9, 2018

10.1K
  • Applied the Ussing chamber/voltage clamp technique to measure short-circuit current (Isc) as an indicator of electrogenic ion transport.
  • Tested the effects of EspP, its neutralized form, and Serine Protease A on Isc in HCM.

    • Main Results:

    • EspP significantly stimulates Isc in HCM, indicating active ion transport.
    • The EspP-stimulated Isc is independent of protease activity and cystic fibrosis transmembrane conductance regulator (CFTR) channel activity.
    • The effect of EspP is partially dependent on extracellular calcium (Ca2+).
    • Serine Protease A also stimulates Isc, but this response is CFTR-dependent.

    Conclusions:

    • EspP acts as an enterotoxin by stimulating colonic, CFTR-independent active ion transport.
    • EspP may play a significant role in the pathophysiology of watery diarrhea caused by EHEC infections.
    • Serine protease toxins secreted by E. coli pathogens represent a potential class of enterotoxins contributing to diarrheal diseases.