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Rapid Generation of Amyloid from Native Proteins In vitro
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Matthew W Turner1,2, Roberto Cruz3, Jordan Elwell4

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Summary
This summary is machine-generated.

Veratrum californicum contains steroidal alkaloids that inhibit the Hedgehog (Hh) signaling pathway. Other alkaloids in the plant, beyond cyclopamine, also contribute to this Hh pathway inhibition.

Keywords:
HPLC-MSShh-Light II cellsVeratrum californicumcyclopaminehedgehog signaling

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Area of Science:

  • Pharmacognosy
  • Molecular Biology
  • Plant Chemistry

Background:

  • Veratrum californicum is a known source of steroidal alkaloids.
  • Cyclopamine, a steroidal alkaloid from V. californicum, inhibits the Hedgehog (Hh) signaling pathway.

Purpose of the Study:

  • To quantitatively analyze the alkaloid composition (cyclopamine, veratramine, muldamine, isorubijervine) in different parts of V. californicum (leaf, stem, root/rhizome).
  • To investigate whether other alkaloids in V. californicum extracts contribute to Hh signaling inhibition.
  • To compare the Hh signaling inhibitory effects of plant extracts with mixtures of purified alkaloids.

Main Methods:

  • Quantitative analysis of specific steroidal alkaloids in V. californicum leaf, stem, and root/rhizome.
  • Replication of alkaloid concentrations found in extracts using commercially available standards.
  • Assay of Hh signaling inhibition using Shh-Light II cells, comparing plant extracts to alkaloid standard mixtures.

Main Results:

  • Alkaloid combinations demonstrated enhanced Hh signaling pathway antagonism compared to cyclopamine alone.
  • Significant differences in Hh pathway inhibition were observed between stem and root/rhizome extracts and their corresponding alkaloid standard mixtures.
  • These findings indicate the presence of additional Hh signaling inhibitory alkaloids in V. californicum extracts.

Conclusions:

  • V. californicum contains multiple steroidal alkaloids that contribute to Hedgehog signaling pathway inhibition.
  • The composition and synergistic effects of these alkaloids vary between plant parts.
  • Further research into these additional alkaloids may reveal novel therapeutic agents targeting the Hh pathway.