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Evaluating the Role of Mitochondrial Function in Cancer-related Fatigue
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Role of

Gang Zhou1, Ying-Si Wang2, Hong Peng3

  • 1Guangdong Open Laboratory of Applied Microbiology, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Guangdong Institute of Microbiology, Guangzhou, Guangdong 510070, China. zgbees@gdim.cn.

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|September 12, 2018
PubMed
Summary
This summary is machine-generated.

The tRNA 2-thiocytidine synthetase (ttcA) gene in Citrobacter werkmanii strain BF-6 is crucial for resistance to 1,2-benzisothiazolin-3-one (BIT) and biofilm formation. Deleting ttcA reduced growth and motility but increased biofilm production.

Keywords:
Citrobacter werkmaniiTn5 mutagenesisbiofilm formationswimming motilityttcA gene

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Isothiazolones are widely used biocides, but resistance mechanisms are not fully understood.
  • Biofilm formation in bacteria like Citrobacter werkmanii can lead to persistent infections and reduced susceptibility to antimicrobial agents.

Purpose of the Study:

  • To identify genes in Citrobacter werkmanii strain BF-6 associated with resistance to 1,2-benzisothiazolin-3-one (BIT).
  • To investigate the role of specific genes in biofilm formation and BIT resistance.

Main Methods:

  • Generation and screening of a Tn5 transposon mutant library of C. werkmanii strain BF-6.
  • Deletion of the tRNA 2-thiocytidine synthetase gene (ttcA) via homologous recombination.
  • Phenotypic analysis of the wild-type and ΔttcA mutant strains, including growth rate, motility, and biofilm formation assays.

Main Results:

  • Three genes involved in BIT resistance were identified.
  • The ΔttcA mutant showed decreased growth rate and swimming motility.
  • The ΔttcA mutant exhibited increased biofilm formation, though BIT resistance was not significantly enhanced.

Conclusions:

  • The ttcA gene plays a significant role in both 1,2-benzisothiazolin-3-one resistance and biofilm formation in Citrobacter werkmanii strain BF-6.
  • This study provides novel insights into the genetic basis of biocide resistance and biofilm development in this bacterium.