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Updated: Feb 5, 2026

Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants
Published on: May 5, 2023
A concise synthesis of peramine, a metabolite of endophytic fungi
Shinsaku Ito1, Yuta Yamamoto1, Toshio Nishikawa1
1a Graduate School of Bioagricultural Sciences , Nagoya University Furo-cho , Nagoya , Japan.
Abstract:
The total synthesis of peramine, a natural product isolated from an endophytic fungi, has been achieved in four steps and 34% overall yield from known compounds. The key step was the one-pot construction of the pyrrolopyrazinone ring from pyrrole amide and propargyl bromide. The preparation of peramine-d4 as an internal standard for quantitative analysis by MS is also described.
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