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Related Experiment Video

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Development of a Larval Zebrafish Infection Model for Clostridioides difficile

Development of a Larval Zebrafish Infection Model for Clostridioides difficile

Published on: February 14, 2020

Association between Clostridioides difficile ribotypes, restriction endonuclease analysis types, and toxin gene

Hiroki Watanabe¹, Yusuke Koizumi¹, Asami Matsumoto²

¹Department of Clinical Infectious Diseases, Aichi Medical University Hospital, 1-1, Yazako-karimata, Nagakute, Aichi 480-1195, Japan; Department of Infection Control and Prevention, Aichi Medical University Hospital, 1-1, Yazako-karimata, Nagakute, Aichi 480-1195, Japan.

|September 12, 2018

Summary

Clostridioides difficile toxin gene expression was not linked to specific ribotypes or restriction endonuclease analysis (REA) types. Binary toxin production also showed no correlation with toxin A (tcdA) and toxin B (tcdB) expression levels.

Keywords:

Clostridioides difficile Gene Restriction endonuclease analysis Ribotype Toxin

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Published on: February 7, 2021

Area of Science:

Microbiology
Infectious Diseases
Molecular Biology

Background:

Clostridioides difficile is a significant cause of severe gastrointestinal infections.
The role of specific C. difficile ribotypes in virulence remains debated.
Understanding toxin gene expression is crucial for assessing C. difficile pathogenicity.

Purpose of the Study:

To investigate the association between C. difficile ribotypes, restriction endonuclease analysis (REA) types, and the expression of key toxin genes.
To determine if molecular strain typing correlates with the levels of toxin A (tcdA), toxin B (tcdB), and binary toxin (cdtB) gene expression.

Main Methods:

Utilized 53 clinical C. difficile isolates.
Performed PCR ribotyping and REA typing to classify strains into distinct molecular types.
Quantified tcdA, tcdB, and cdtB gene expression using real-time PCR, normalized to the housekeeping gene rpoA.

Main Results:

All strains expressed tcdB, while tcdA was expressed in 48/53 strains and cdtB in 4/53 strains.
Toxin gene expression levels (tcdA, tcdB, cdtB) did not significantly differ based on ribotype or REA type.
Binary toxin (cdtB) expression was not correlated with tcdA and tcdB expression levels.

Conclusions:

Ribotype and REA type are not reliable indicators of C. difficile toxin gene expression.
The production of binary toxin is independent of tcdA and tcdB expression levels in the studied strains.
Findings suggest that virulence factors beyond ribotype and toxin gene expression may influence C. difficile pathogenicity.