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Federica Giovanelli1, Matteo Mattellini2, Gianluca Fichi3

  • 1Dipartimento di Scienze Veterinarie, University of Pisa, Viale delle Piagge 2, 56124 Pisa, Italy. federica.giovanelli76@gmail.com.

Veterinary Sciences
|September 12, 2018
PubMed
Summary

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Plant compounds like rutin and mangiferin show promising in vitro anthelmintic activity against sheep gastrointestinal strongyles (GIS). These natural compounds effectively inhibit egg hatching and larval development, offering potential alternatives to synthetic drugs.

Area of Science:

  • Veterinary Parasitology
  • Natural Product Chemistry
  • Pharmacology

Background:

  • Sheep gastrointestinal strongyles (GIS) pose a significant threat to livestock health and productivity.
  • Anthelmintic resistance necessitates the exploration of novel control strategies.
  • Plant-derived compounds offer a potential source of new anthelmintic agents.

Purpose of the Study:

  • To investigate the in vitro anthelmintic activity of four plant-derived compounds: mangiferin, rutin, quercetin, and β-sitosterol.
  • To evaluate the efficacy of these compounds against sheep GIS using egg hatch, larval development, and larval mortality assays.
  • To compare the activity of these natural compounds with a standard synthetic anthelmintic, thiabendazole (TBZ).

Main Methods:

  • In vitro anthelmintic assays including egg hatch test (EHT), larval development test (LDT), and larval mortality/paralysis test (LMT).
Keywords:
anthelmintic activitygastrointestinal strongylesin vitromangiferinquercetinrutinsheepβ-sitosterol

Related Experiment Videos

  • Testing of mangiferin, rutin, quercetin, and β-sitosterol at various concentrations against sheep GIS.
  • Statistical analysis using the χ² test and calculation of EC50 values.

    • Main Results:

    • All tested plant compounds significantly inhibited egg hatching compared to untreated controls.
    • Rutin, mangiferin, and β-sitosterol demonstrated significant inhibition of larval development (L1 to L3).
    • Rutin and mangiferin showed significant larval mortality comparable to TBZ, while β-sitosterol had limited efficacy in the mortality test.

    Conclusions:

    • Mangiferin and rutin exhibit significant in vitro anthelmintic properties against sheep GIS, comparable to thiabendazole in certain assays.
    • These plant-derived compounds represent potential candidates for developing new natural anthelmintics to combat GIS infections in sheep.
    • Further research is warranted to explore the in vivo efficacy and mechanisms of action of these promising natural compounds.