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Updated: Feb 5, 2026

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
Published on: May 24, 2017
COMRADES determines in vivo RNA structures and interactions
Omer Ziv1,2, Marta M Gabryelska3, Aaron T L Lun4
1Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, UK. omer.ziv@gurdon.cam.ac.uk.
Abstract:
The structural flexibility of RNA underlies fundamental biological processes, but there are no methods for exploring the multiple conformations adopted by RNAs in vivo. We developed cross-linking of matched RNAs and deep sequencing (COMRADES) for in-depth RNA conformation capture, and a pipeline for the retrieval of RNA structural ensembles. Using COMRADES, we determined the architecture of the Zika virus RNA genome inside cells, and identified multiple site-specific interactions with human noncoding RNAs.
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