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Overview of Fatty Acid Metabolism01:28

Overview of Fatty Acid Metabolism

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Lipids also are sources of energy that power cellular processes. Like carbohydrates, lipids are composed of carbon, hydrogen, and oxygen, but these atoms are arranged differently. Most lipids are nonpolar and hydrophobic. Major types include fats and oils, waxes, phospholipids, and steroids.
Fatty acids are catabolized in a process called beta-oxidation, which takes place in the matrix of the mitochondria and converts their fatty acid chains into two-carbon units of acetyl groups. The acetyl...
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Amino Acid Biosynthetic Pathways01:29

Amino Acid Biosynthetic Pathways

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Amino acid biosynthesis is essential for cell growth, protein synthesis, and metabolic regulation. Cells generate essential and non-essential amino acids from metabolic intermediates to sustain vital biological functions. These intermediates originate from key metabolic pathways: glycolysis, the tricarboxylic acid (TCA) cycle, and the pentose phosphate pathway. Important precursors include α-ketoglutarate, pyruvate, oxaloacetate, phosphoenolpyruvate, and erythrose-4-phosphate, which...
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C4 Pathway and CAM01:27

C4 Pathway and CAM

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Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
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Synthesis and Decomposition Reactions02:17

Synthesis and Decomposition Reactions

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Synthesis and decomposition are two types of redox reactions. Synthesis means to make something, whereas decomposition means to break something. The reactions are accompanied by chemical and energy changes. 
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Amino acids03:42

Amino acids

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Amino acids are the monomers that comprise proteins. Each amino acid has the same fundamental structure, which consists of a central carbon atom, or the alpha (α) carbon, bonded to an amino group (NH2), a carboxyl group (COOH), and to a hydrogen atom. Every amino acid also has another atom or group of atoms bonded to the central atom known as the R group. There are 20 common amino acids present in proteins, each with a different R group. Variation in the amino acid sequence is responsible for...
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Transfer RNA Synthesis02:36

Transfer RNA Synthesis

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One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
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Related Experiment Video

Updated: Feb 5, 2026

Identification of Fatty Acids in Bacillus cereus
08:41

Identification of Fatty Acids in Bacillus cereus

Published on: December 5, 2016

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Engineering the fatty acid synthesis pathway in

María Santos-Merino1, M Pilar Garcillán-Barcia1, Fernando de la Cruz1

  • 1Instituto de Biomedicina y Biotecnología de Cantabria (Universidad de Cantabria-Consejo Superior de Investigaciones Científicas), Santander, Cantabria Spain.

Biotechnology for Biofuels
|September 12, 2018
PubMed
Summary

Cyanobacteria can be genetically engineered to enhance fatty acid production for renewable energy. Specific gene modifications in Synechococcus elongatus PCC 7942 significantly increased omega-3 fatty acid yields.

Keywords:
CyanobacteriaFatty acid synthesisOmega-3 fatty acidsSynechococcus elongatus PCC 7942fab genes

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Area of Science:

  • Microbiology
  • Biotechnology
  • Synthetic Biology

Background:

  • Cyanobacteria are promising for microbial fatty acid production due to photosynthesis and CO2 fixation.
  • Fatty acid synthesis pathways in cyanobacteria are not fully understood.
  • Engineering these pathways can yield valuable products like fatty acids for renewable energy.

Purpose of the Study:

  • To investigate the function of saturated fatty acid synthesis enzymes in cyanobacteria.
  • To genetically modify Synechococcus elongatus PCC 7942 to alter its fatty acid profile.
  • To enhance the production of specific fatty acids, such as alpha-linolenic acid.

Main Methods:

  • Genetic engineering of Synechococcus elongatus PCC 7942.
  • Overexpression and deletion of genes encoding fatty acid synthesis enzymes.
  • Analysis of lipid profiles in engineered mutants.
  • Introduction of desaturase genes (desA, desB) from Synechococcus sp. PCC 7002.

Main Results:

  • Genetic modifications significantly altered the fatty acid composition of S. elongatus PCC 7942.
  • A specific mutant (fabF overexpression, fadD deletion, desA/desB overexpression) showed the highest alpha-linolenic acid production.
  • Observed changes in fatty acid composition differed from patterns seen in Escherichia coli.

Conclusions:

  • The fatty acid synthesis pathway in cyanobacteria is amenable to genetic manipulation.
  • Engineered cyanobacteria can be used to improve omega-3 fatty acid production.
  • This study offers insights into saturated fatty acid synthesis and strategies for cyanobacterial lipid manipulation.