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Updated: Feb 5, 2026

One-channel Cell-attached Patch-clamp Recording
Published on: June 9, 2014
Patch-Clamp Recording of the CRAC Channel Current in STIM-Orai Overexpressing Cells
Yiming Zhang1, Sisi Zheng1, Yubin Zhou2
1Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing, China.
Abstract:
Induced by the depletion of ER calcium store, the calcium influx through calcium release-activated calcium (CRAC) channels is an ubiquitous way of Ca2+ influx for most cell types. This process is mediated by STIM protein, ER calcium sensor, and PM localized Orai calcium channels. Biophysical characterization of this STIM-Orai-mediated current, or ICRAC, with whole-cell patch-clamp technique is essential for revealing the molecular mechanisms underlying the process of STIM-Orai activation or modulation. Here we describe one commonly used procedure of monitoring CRAC activity with whole-cell patch-clamp technique.
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