Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Energy Stored in a Capacitor01:12

Energy Stored in a Capacitor

4.7K
When an archer pulls the string in a bow, he saves the work done in the form of elastic potential energy. When he releases the string, the potential energy is released as kinetic energy of the arrow. A capacitor works on the same principle in which the work done is saved as electric potential energy. The potential energy (UC) could be calculated by measuring the work done (W) to charge the capacitor.
4.7K
Energy Stored in Capacitors01:10

Energy Stored in Capacitors

1.1K
A parallel plate capacitor, when connected to a battery, develops a potential difference across its plates. This potential difference is key to the operation of the capacitor, as it determines how much electrical energy the capacitor can store.
By integrating the equation that relates voltage and current in a capacitor, one can derive an equation for the voltage across the capacitor at any given time. This equation is crucial in understanding and predicting the behavior of capacitors in...
1.1K
Energy Stored in Inductors01:16

Energy Stored in Inductors

959
An inductor is ingeniously crafted to accumulate energy within its magnetic field. This field is a direct result of the current that meanders through its coiled structure. When this current maintains a steady state, there is no detectable voltage across the inductor, prompting it to mimic the behavior of a short circuit when faced with direct current.
In terms of gauging the energy stored within an inductor, it is equivalent to the integral of the power delivered at every individual moment, all...
959
Energy Stored in a Capacitor: Problem Solving01:26

Energy Stored in a Capacitor: Problem Solving

1.8K
In 1749, Benjamin Franklin coined the word battery for a series of capacitors connected to store energy. Capacitors store electric potential energy that can be released over a short time. This property means capacitors have a wide range of applications.
Capacitor-discharge ignition is a type of ignition system commonly found in small engines where the energy released from a capacitor ignites an induction coil that, in turn, fires the spark plug.
To calculate the energy stored in a capacitor of...
1.8K
Energy Stored In A Coaxial Cable01:31

Energy Stored In A Coaxial Cable

2.1K
A coaxial cable consists of a central copper conductor used for transmitting signals, followed by an insulator shield, a metallic braided mesh that prevents signal interference, and a plastic layer that encases the entire assembly.
In the simplest form, a coaxial cable can be represented by two long hollow concentric cylinders in which the current flows in opposite directions. The magnetic field inside and outside the coaxial cable is determined by using Ampère's law. The magnetic field inside...
2.1K
Operational Amplifiers01:17

Operational Amplifiers

2.0K
The operational amplifier, often referred to as an op-amp, is a multifaceted building block of a circuit. This electronic component functions like a voltage-controlled voltage source and can also be used to create a voltage- or current-controlled current source. The design of an operational amplifier enables it to execute mathematical operations when external components like resistors and capacitors are linked to its terminals. An op-amp has the capacity to sum signals, amplify a signal,...
2.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A growing role for ER-Ca<sup>2+</sup> release and store-operated Ca<sup>2+</sup> entry in neuronal physiology and pathophysiology.

Current opinion in neurobiology·2026
Same author

Synergism of IP3R and Parkin mutants identifies mitochondrial stress as an early feature of Parkinson's disease.

Disease models & mechanisms·2025
Same author

Lysosomal TPC2 channels disrupt Ca2+ entry and dopaminergic function in models of LRRK2-Parkinson's disease.

The Journal of cell biology·2025
Same author

Altered Mitochondrial Bioenergetics and Calcium Kinetics in Young-Onset PLA2G6 Parkinson's Disease iPSCs.

Journal of neurochemistry·2025
Same author

Erratum to: Distinct regulation of bioenergetics and translation by group I mGluR and NMDAR.

EMBO reports·2024
Same author

Corrigendum to "Generation of feeder-independent transgene-free iPSC lines from a young-onset Parkinson's disease (YOPD) patient with a homozygous PLA2G6: c.2222G>A (p. Arg741Gln) mutation (NCBSi003-A) and unaffected heterozygous parent (NCBSi004-A)" [Stem Cell Res. 67 (2023) 103033].

Stem cell research·2024

Related Experiment Video

Updated: Feb 5, 2026

Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement
10:45

Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement

Published on: July 26, 2017

10.7K

Store-Operated Ca

Sumita Chakraborty1,2, Gaiti Hasan3

  • 1National Centre for Biological Sciences, TIFR, Bangalore, India.

Methods in Molecular Biology (Clifton, N.J.)
|September 12, 2018
PubMed
Summary
This summary is machine-generated.

This study details methods for measuring calcium (Ca2+) signaling in Drosophila neurons. It focuses on inositol 1,4,5-trisphosphate (IP3)-mediated release and store-operated Ca2+ entry (SOCE) pathways.

Keywords:
CarbacholFluo-4IP3RIndo-1OraiSTIMThapsigarginTime-lapse imaging

More Related Videos

Using a Virtual Store As a Research Tool to Investigate Consumer In-store Behavior
09:17

Using a Virtual Store As a Research Tool to Investigate Consumer In-store Behavior

Published on: July 24, 2017

11.9K
Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber
14:18

Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber

Published on: February 13, 2012

21.8K

Related Experiment Videos

Last Updated: Feb 5, 2026

Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement
10:45

Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement

Published on: July 26, 2017

10.7K
Using a Virtual Store As a Research Tool to Investigate Consumer In-store Behavior
09:17

Using a Virtual Store As a Research Tool to Investigate Consumer In-store Behavior

Published on: July 24, 2017

11.9K
Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber
14:18

Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber

Published on: February 13, 2012

21.8K

Area of Science:

  • Neuroscience
  • Cell Biology
  • Biochemistry

Background:

  • Neuronal intracellular calcium signals are crucial for neurotransmission and are often initiated by G protein-coupled receptors (GPCRs).
  • GPCR activation leads to the production of inositol 1,4,5-trisphosphate (IP3), which triggers calcium release from the endoplasmic reticulum (ER) via IP3 receptors (IP3Rs).
  • Following ER calcium depletion, store-operated calcium entry (SOCE) occurs, mediated by STIM and Orai proteins, to replenish ER calcium stores.

Purpose of the Study:

  • To describe standardized protocols for measuring calcium signaling pathways in primary cultures of Drosophila neurons.
  • To facilitate the study of IP3-mediated calcium release and SOCE in neuronal function.
  • To leverage Drosophila's genetic tractability for investigating neuronal calcium dynamics.

Main Methods:

  • Utilizing neuron-specific knockdown in Drosophila for genetic manipulation of calcium signaling proteins.
  • Standardizing primary neuronal culture techniques from dissected Drosophila brains.
  • Employing calcium imaging with indicator dyes to measure calcium dynamics.

Main Results:

  • Established protocols for measuring IP3-mediated calcium release from ER stores.
  • Developed methods for assessing passive depletion of ER calcium stores.
  • Standardized procedures for quantifying store-operated calcium entry (SOCE) in cultured Drosophila neurons.

Conclusions:

  • The described protocols enable robust calcium measurements in Drosophila neurons.
  • These methods are essential for dissecting the roles of IP3R and SOCE in neuronal calcium signaling.
  • The study provides a foundation for further genetic and physiological investigations of neuronal calcium homeostasis in Drosophila.