Related Experiment Video
Updated: Feb 5, 2026

Measuring Fast Calcium Fluxes in Cardiomyocytes
Published on: November 29, 2011
Measurement of the CRAC Channel Fast Ca
1School of Medicine, University of Adelaide, South Australian Health and Medical Research Institute (SAHMRI), Adelaide, SA, Australia. grigori.rychkov@adelaide.edu.au.
Abstract:
Fast Ca2+-dependent inactivation (FCDI) is a safety mechanism limiting Ca2+ entry through some types of Ca2+ channels, including Ca2+ release-activated Ca2+ (CRAC) channels. This type of inactivation is caused by Ca2+, which passes through Ca2+ channel and binds to a specific site within a short distance from the inner mouth of the pore, causing channel to shut.The main technique that is used to investigate FCDI is whole-cell patch clamping. Since the cloning of the molecular components of the CRAC channel, STIM1 and Orai1, FCDI of CRAC channel has been studied using HEK293T heterologous expression system. In this paper we describe a method of quantifying CRAC channel FCDI by using instantaneous tail currents.
Related Concept Videos
Ion Channels
Ion channels are specialized integral membrane proteins on the plasma membrane that allow...
Fast Fourier Transform
The computational efficiency of the FFT becomes...
Channel Rhodopsins
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...
Fast Decoupled and DC Powerflow
Gene Evolution - Fast or Slow?
In contrast, regions which code...
Gene Evolution - Fast or Slow?

