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High-Resolution Imaging of STIM/Orai Subcellular Localization Using Array Confocal Laser Scanning Microscopy.

Andras T Deak1,2, Benjamin Gottschalk1, Emrah Eroglu1

  • 1Molecular Biology and Biochemistry, Gottfried Schatz Research Center, Medical University of Graz, Graz, Austria.

Methods in Molecular Biology (Clifton, N.J.)
|September 12, 2018
PubMed
Summary

This study visualizes the dynamics of STIM1 and Orai1 proteins, key players in calcium entry, using fluorescent protein chimeras and advanced microscopy. It offers insights into their real-time subcellular movements during cellular calcium signaling.

Keywords:
Ca2+ signalingCell transfectionFluorescence microscopyFluorescent proteinsGenetically encoded toolsImage analysisLive-cell imaging

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