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Related Experiment Video

Updated: Feb 5, 2026

Label-free Single Molecule Detection Using Microtoroid Optical Resonators
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Single-Channel Single-Molecule Detection (SC-SMD) System.

Laura G Ceballos1, Alexander Asanov2, Luis Vaca3

  • 1Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Coyoacán, Mexico DF, Mexico.

Methods in Molecular Biology (Clifton, N.J.)
|September 12, 2018
PubMed
Summary

Single-molecule fluorescence imaging combined with electrophysiology offers a powerful method to study ionic channel stoichiometry and function. This approach enables simultaneous patch clamping and single-molecule measurements for detailed channel analysis.

Keywords:
Patch clampSingle channelSingle moleculeTRPC channels

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Area of Science:

  • Biophysics
  • Molecular Biology
  • Neuroscience

Background:

  • Ionic channels are crucial for cellular function.
  • Understanding their stoichiometry and function is essential.
  • Current methods may not offer simultaneous functional and molecular insights.

Purpose of the Study:

  • To describe a typical Surface-Correlated Single-Molecule Detection (SC-SMD) experiment.
  • To detail the integration of electrophysiology with single-molecule imaging.
  • To enable simultaneous determination of ionic channel properties.

Main Methods:

  • Preparation of plasmids encoding fluorescently tagged ionic channels.
  • Utilizing the SC-SMD system for combined measurements.
  • Performing simultaneous patch clamping and single-molecule fluorescence imaging.

Main Results:

  • Demonstration of a feasible SC-SMD experimental workflow.
  • Simultaneous acquisition of electrophysiological and single-molecule data.
  • Provides insights into ionic channel stoichiometry and function.

Conclusions:

  • SC-SMD is a powerful technique for studying ionic channels.
  • This method allows for simultaneous functional and molecular characterization.
  • Facilitates a deeper understanding of ion channel behavior.