Development of Activity-Based Proteomic Probes for Protein Citrullination
Venkatesh V Nemmara1,2, Paul R Thompson3,4
1Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, LRB 826, 364 Plantation Street, Worcester, MA, 01605, USA.
Current Topics in Microbiology and Immunology
|September 12, 2018
Summary
Activity-based protein profiling (ABPP) probes are key tools for studying protein arginine deiminases (PADs) and their role in inflammatory diseases and cancer. This review highlights ABPP development for PADs and their inhibitors.
Area of Science:
- Biochemistry
- Molecular Biology
- Chemical Biology
Background:
- Protein arginine deiminases (PADs) catalyze peptidyl arginine deimination to peptidyl citrulline.
- Increased citrullination is linked to inflammatory diseases and cancers.
- PADs influence critical cellular processes like apoptosis and gene transcription.
Purpose of the Study:
- To review the development of activity-based protein profiling (ABPP) probes targeting PADs.
- To explore the utility of ABPPs in understanding PAD function and citrullination in pathology.
- To highlight ABPPs as a platform for discovering PAD inhibitors.
Main Methods:
- Development and application of activity-based protein profiling (ABPP) probes.
- Review of PAD structure, function, and signaling pathways.
- Analysis of recent advances in PAD inhibitor discovery.
Main Results:
- ABPP probes have been successfully developed to target PADs.
- ABPPs enable in vivo studies of PAD activity and citrullination.
- ABPPs serve as a valuable platform for high-throughput screening of PAD inhibitors.
Conclusions:
- ABPPs are essential tools for investigating PADs in biological systems.
- Understanding PADs and citrullination is crucial for disease research.
- Continued development of PAD-targeted ABPPs and inhibitors holds therapeutic potential.
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