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Activity-Based Protein Profiling at the Host-Pathogen Interface.

Yekaterina Kovalyova1,2, Stavroula K Hatzios3,4,5

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Current Topics in Microbiology and Immunology
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Summary
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Activity-based protein profiling (ABPP) uses chemical probes to identify active enzymes in infections. This functional proteomic approach reveals how microbial enzymes impact host immunity and pathogenicity.

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Area of Science:

  • Biochemistry
  • Microbiology
  • Proteomics

Background:

  • Activity-based protein profiling (ABPP) is a chemical proteomic strategy.
  • ABPP utilizes chemical probes to identify reactive amino acids within proteins.
  • This technique allows for the selective detection of active enzymes in complex biological samples.

Purpose of the Study:

  • To apply ABPP for global profiling of enzymes at the host-pathogen interface.
  • To understand the role of enzyme activity in microbial infections.
  • To investigate mechanisms of microbial pathogenicity, host immunity, and metabolic adaptation.

Main Methods:

  • Employing chemical probes targeting catalytically active enzymes.
  • Analyzing functional proteomes in various infection models, from in vitro co-cultures to animal models.
  • Utilizing ABPP to map enzyme activity in host-pathogen interactions.

Main Results:

  • ABPP successfully identified enzymes active at the host-pathogen interface.
  • Revealed enzyme-mediated mechanisms contributing to microbial pathogenicity.
  • Demonstrated the role of enzymes in host immune responses and pathogen metabolic adaptation during infection.

Conclusions:

  • ABPP is a powerful tool for dissecting functional proteomes during infection.
  • Enzyme activity plays a critical role in shaping host-pathogen dynamics.
  • This approach provides insights into infection pathogenesis and host-pathogen interactions.