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The effects of apheresis, storage time, and leukofiltration on microparticle formation in apheresis platelet products

Ming Gao1, Bin Zhang1, Yue Zhang1

  • 1Department of Transfusion, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.

Transfusion
|September 12, 2018
PubMed
Abstract

Insights

Platelet apheresis affects microparticle (MP) levels, increasing total and platelet-derived MPs while decreasing other types. Storage further elevates MP counts, but leukofiltration shows no significant impact on MP levels in platelet products.

Area of Science:

  • Transfusion Medicine
  • Hematology
  • Biotechnology

Background:

  • Platelet microparticles (PMPs) are abundant in platelet (PLT) products.
  • The impact of PLT apheresis, storage, and leukofiltration on various microparticle (MP) types remains unclear.

Purpose of the Study:

  • To investigate the effects of PLT apheresis, storage duration, and leukofiltration on different MP populations.

Main Methods:

  • Apheresis PLTs, with or without leukofiltration, were stored for 5 days.
  • PLT-poor plasma was analyzed for MPs using flow cytometry, with specific markers for platelet (CD41a), red blood cell (CD235), leukocyte (CD45), monocyte (CD14), and endothelial cell (CD144) derived MPs.

Main Results:

  • Apheresis increased total MPs (TMPs) and PMPs but decreased red blood cell-derived MPs (RMPs), leukocyte-derived MPs (LMPs), and monocyte-derived MPs (MMPs).
  • Storage led to significantly higher TMP, PMP, and RMP counts in supernatants by Day 3 and Day 5.
  • Leukofiltration did not significantly alter MP levels in PLT products.

Conclusions:

  • The apheresis procedure influences MP formation, reducing RMPs, LMPs, and MMPs.
  • Microparticle levels, including TMPs, PMPs, RMPs, LMPs, and MMPs, increase in PLT supernatants during storage.
  • Leukofiltration has no significant effect on the levels of various MPs in PLT products.

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