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Published on: September 5, 2025
Updated protocols for the detection of Sotatercept and Luspatercept in human serum
Christian Reichel1,2, Günter Gmeiner1, Katja Walpurgis3
1Doping Control Laboratory Seibersdorf, Seibersdorf Labor GmbH, A-2444, Seibersdorf, Austria.
Abstract:
We recently published two protocols for the detection of Sotatercept (ACE-011, ACVR2A-Fc) and Luspatercept (ACE-536, ACVR2B-Fc) in human serum. Both methods used covalently immobilized antibodies on agarose beads for immunoprecipitation and SAR-PAGE/Western blotting for detection. Disadvantages were the relatively high amount of antibody required per sample (10 μg) and the need of a secondary antibody for the final detection. The updated protocols overcome these limitations by antigen-antibody complex formation in solution followed by capture of the complex with anti-antibody-coated magnetic beads. They also omit the secondary antibody incubation step by usage of biotinylated primary antibodies, which can be directly incubated with streptavidin-HRP. Thus, the new protocols are faster, simpler, and cheaper and offer comparable sensitivities.
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