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Sphingomonas floccifaciens sp. nov., isolated from subterranean sediment
Qiu-Ming Fan1, Ren-Gang Zhang1, Han-Yi Chen1
1College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
International Journal of Systematic and Evolutionary Microbiology
|September 12, 2018
Summary
A novel orange-pigmented bacterium, Sphingomonas floccifaciens sp. nov., was discovered in Chinese permafrost sediments. This Gram-negative bacterium expands the known diversity within the Sphingomonas genus.
Area of Science:
- Microbiology
- Bacteriology
- Genomics
Background:
- Permafrost environments harbor unique microbial communities.
- The genus Sphingomonas is known for its metabolic versatility and presence in diverse environments.
Purpose of the Study:
- To isolate and characterize a novel bacterium from a subterranean sediment sample in China's Mohe permafrost area.
- To determine the phylogenetic and phenotypic characteristics of the novel isolate to establish its taxonomic position.
Main Methods:
- Isolation and cultivation of bacteria from permafrost sediment.
- Gram staining, motility, and sporulation tests.
- Optimal growth condition determination (temperature, pH, NaCl).
- Phylogenetic analysis using 16S rRNA gene sequences.
- DNA G+C content determination.
- Analysis of major ubiquinone, fatty acids, polar lipids, and polyamines.
Main Results:
- A Gram-negative, non-motile, non-sporulating, rod-shaped, orange-pigmented bacterium, strain FQM01T, was isolated.
- Strain FQM01T exhibited optimal growth at 25°C, pH 7.0, and 0% NaCl.
- Phylogenetic analysis placed strain FQM01T within the genus Sphingomonas, with Sphingomonas spermidinifaciens GDMCC 1.657T as the closest relative (97.6% similarity).
- The DNA G+C content was 66.9 mol%.
- Detailed chemotaxonomic analyses (ubiquinone, fatty acids, polar lipids, polyamines) supported its distinct identity.
Conclusions:
- Strain FQM01T represents a novel species within the genus Sphingomonas.
- The proposed name for this new species is Sphingomonas floccifaciens sp. nov.
- The type strain is FQM01T, with accession numbers CGMCC 1.15797T and KCTC 52630T.
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