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Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR
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Unique Circulating MicroRNA Profiles in HIV Infection
Venkata Narla1, Nirav Bhakta2, Jane E Freedman3
1Division of Cardiology and the Center of Excellence in Vascular Research, San Francisco General Hospital, University of California, San Francisco, CA.
Journal of Acquired Immune Deficiency Syndromes (1999)
|September 12, 2018
Summary
Extracellular microRNAs (miRNAs) show distinct expression patterns in individuals with HIV infection. These miRNA signatures may aid in understanding HIV control and associated cardiovascular conditions.
Area of Science:
- Molecular Biology
- Immunology
- Virology
Background:
- MicroRNAs (miRNAs) are key regulators of gene expression.
- Extracellular miRNAs are increasingly recognized as potential biomarkers.
- Understanding their role in HIV infection is crucial.
Purpose of the Study:
- To investigate the association between extracellular miRNAs and HIV infection.
- To identify specific miRNA expression profiles in HIV-infected individuals.
Main Methods:
- A cross-sectional study analyzed plasma-derived miRNAs in 69 HIV-infected individuals and 24 controls.
- High-throughput Real-Time PCR (Fluidigm) was used to assay 192 miRNAs.
- False discovery rate (FDR) was applied for statistical analysis.
Main Results:
- Twenty-nine miRNAs were differentially expressed in HIV-infected individuals compared to controls.
- Thirty-four miRNAs were differentially expressed in elite controllers versus HIV-negative subjects.
- Specific miRNAs identified have known roles in HIV latency and coronary artery disease.
Conclusions:
- Unique miRNA expression profiles were identified in HIV+ subjects and elite controllers.
- These miRNA signatures may elucidate mechanisms of viral and immunological control.
- Potential diagnostic or prognostic value for HIV-associated coronary artery disease is suggested.
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