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The ITS2 Database
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Development of a curated Hershberger database.

P Browne1, N C Kleinstreuer2, P Ceger3

  • 1OECD/EHS, Paris, France.

Reproductive Toxicology (Elmsford, N.Y.)
|September 12, 2018
PubMed
Summary

This review identified reference chemicals for validating alternative testing methods. It found disagreements in Hershberger bioassay results and with other in vivo studies, highlighting the need for reliable chemical screening.

Keywords:
AndrogenicAnti-androgenicDisruptionEDSPEndocrineHershbergerMale reproductionReference chemicalSystematic review

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Area of Science:

  • Toxicology
  • Endocrinology
  • Chemical Safety Assessment

Background:

  • The Hershberger bioassay is a key in vivo method for assessing androgenic activity.
  • Accurate chemical screening is crucial for regulatory safety assessments and validating new testing strategies.
  • Existing data on chemical androgenic responses require systematic evaluation for reliability.

Purpose of the Study:

  • To systematically review Hershberger bioassay data for a large set of chemicals.
  • To evaluate the concordance of Hershberger assay results with other in vivo rodent studies.
  • To identify reliable reference chemicals for validating alternative methods for endocrine disruption screening.

Main Methods:

  • Conducted a systematic literature review to identify Hershberger bioassays for approximately 3200 chemicals.
  • Extracted experimental results for 134 chemicals into a database.
  • Characterized uncertainty and evaluated concordance with other in vivo rodent studies measuring androgen-responsive endpoints.

Main Results:

  • 28% of chemicals tested in multiple Hershberger studies showed disagreements (i.e., both positive and negative results).
  • 43% of chemicals tested in both Hershberger and other in vivo studies showed disagreements in androgen-responsive endpoints.
  • 49 chemicals were identified with reproducible androgen pathway responses across multiple in vivo rodent studies.

Conclusions:

  • Significant discordance exists within Hershberger bioassay data and between Hershberger and other in vivo studies.
  • Identified 49 chemicals with reproducible androgenic responses, suitable as reference chemicals for method validation.
  • The findings support the development and validation of alternative methods for endocrine activity screening.