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Updated: Feb 5, 2026
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Customization of Aspergillus niger Morphology Through Addition of Talc Micro Particles
Published on: March 15, 2012
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Malic acid production from biodiesel derived crude glycerol using morphologically controlled Aspergillus niger in
J Iyyappan1, G Baskar2, B Bharathiraja1
1Vel Tech High Tech Dr. Rangarajan Dr. Sakunthala Engineering College, Chennai 600062, India.
Bioresource Technology
|September 12, 2018
Summary
Optimizing Aspergillus niger PJR1 fermentation using crude glycerol from biodiesel enhances malic acid production. Dispersed fungal mycelium and controlled conditions yield significantly higher biomass and malic acid.
Area of Science:
- Biotechnology
- Industrial Microbiology
- Biochemical Engineering
Background:
- Crude glycerol, a byproduct of biodiesel production, is an abundant feedstock.
- Aspergillus niger is a key microorganism for organic acid production.
- Optimizing fermentation conditions is crucial for maximizing product yield.
Purpose of the Study:
- To investigate the effects of various parameters on malic acid production by Aspergillus niger PJR1.
- To determine optimal conditions for enhanced biomass and malic acid yield using crude glycerol.
- To explore morphological control of fungal fermentation for improved efficiency.
Main Methods:
- Fermentation experiments were conducted using Aspergillus niger PJR1.
- Crude glycerol concentration, spore inoculum, yeast extract, and shaking frequency were varied.
- Morphological changes (dispersed mycelium vs. pellet) and biomass were analyzed.
- Malic acid yield was quantified under optimized batch fermentation conditions.
Main Results:
- Dispersed fungal mycelium fermentation yielded higher biomass (20.25 ± 0.91 g/L) compared to pellet fermentation.
- Dry cell weight was 21.28% higher in dispersed fermentation.
- Optimal conditions included 160 g/L crude glycerol and 1.5 g/L yeast extract.
- A malic acid yield of 83.23 ± 1.86 g/L was achieved after 192 hours at 25°C.
Conclusions:
- Morphological control of Aspergillus niger fermentation is an effective strategy for increasing malic acid production.
- Crude glycerol from biodiesel production can be efficiently utilized as a feedstock.
- Optimized fermentation parameters significantly enhance malic acid yield.
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