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Related Experiment Videos

Weimin Wang1,2,3, Yan Zhou1,2, Qiang Yao2

  • 1Institute of Combining Chinese Traditional and Western Medicine, Medical College, Yangzhou University, Yangzhou, Jiangsu, China.

Anti-Cancer Agents in Medicinal Chemistry
|September 13, 2018
PubMed
Summary

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Celastrus orbiculatus extract (COE) enhances cisplatin

Area of Science:

  • Oncology
  • Pharmacology
  • Traditional Chinese Medicine

Background:

  • Cisplatin resistance is a major cause of treatment failure in cancer therapy.
  • Combining cisplatin with other agents is a promising strategy to overcome resistance.
  • Celastrus orbiculatus ethyl acetate extract (COE) shows antitumor activity in gastric cancer.

Purpose of the Study:

  • To investigate if COE can increase the sensitivity of gastric cancer cells to cisplatin.
  • To explore the underlying mechanisms of COE's effect on cisplatin sensitivity.

Main Methods:

  • Cell viability was assessed using the CCK8 assay.
  • Apoptosis was analyzed via propidium iodide (PI) and Annexin V/PI staining using flow cytometry.
  • Western blotting was employed to examine caspase signaling pathway proteins.
Keywords:
CCK8Celastrus Orbiculatusapoptosiscaspase pathwaycisplatingastric cancerwestern blotting.

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  • In vivo efficacy was evaluated using a xenograft model.

    • Main Results:

    • COE and cisplatin exhibited synergistic cytotoxicity against gastric cancer cells (BGC 823, SGC 7901) in a dose-dependent manner.
    • COE increased cisplatin-induced apoptosis in vitro.
    • COE enhanced the activation of caspase-8, caspase-9, caspase-3, and PARP1 signaling pathways.
    • In vivo studies confirmed that COE potentiated cisplatin's antitumor effect.

    Conclusions:

    • COE significantly increases the sensitivity of gastric cancer to cisplatin.
    • COE enhances the antitumor efficacy of cisplatin through the modulation of apoptosis and caspase signaling pathways.
    • These findings support COE as a potential adjuvant therapy to overcome cisplatin resistance in gastric cancer.