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Updated: Feb 5, 2026

Single-cell Photoconversion in Living Intact Zebrafish
Published on: March 19, 2018
Protein fishing from single live cells
Elaheh Shekaramiz1, Rupak Doshi2,3, H Kumar Wickramasinghe4,5
1Department of Biomedical Engineering, University of California Irvine, Irvine, CA, USA.
Abstract:
Intracellular protein and proteomic studies using mass spectrometry, imaging microscopy, flow cytometry, or western blotting techniques require genetic manipulation, cell permeabilization, and/or cell lysis. We present a biophysical method that employs a nanoaspirator to 'fish' native cytoplasmic or nuclear proteins from single mammalian cells, without compromising cell viability, followed by ex cellulo quantitative detection. Our work paves the way for spatiotemporally-controlled, quantitative, live, single-cell proteomics.
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