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Related Experiment Video

Updated: Feb 5, 2026

Assays for Validating Histone Acetyltransferase Inhibitors

Assays for Validating Histone Acetyltransferase Inhibitors

Published on: August 6, 2020

Validation of a Modified ANSR® for

Susan Alles¹, Brooke Roman¹, Quynh-Nhi Le¹

¹Neogen Corporation, 620 Lesher Pl, Lansing, MI 48912.

Journal of AOAC International

|September 13, 2018

Summary

The modified ANSR method accurately detects Escherichia coli O157:H7 in various food matrices. This validated assay demonstrates robustness, stability, and comparable performance to reference methods, ensuring reliable food safety testing.

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Area of Science:

Food Microbiology
Molecular Diagnostics
Nucleic Acid Amplification

Background:

The ANSR method utilizes isothermal nucleic acid amplification technology.
Assay modifications enhance sensitivity and internal positive control robustness.

Purpose of the Study:

Validate a modified ANSR method for detecting Escherichia coli O157:H7.
Assess the method's performance against established reference procedures.

Main Methods:

Inclusivity and exclusivity testing with multiple bacterial strains.
Matrix testing using beef trim, ground beef, spinach, and water.
Robustness, stability, and independent laboratory validation studies were performed.

Main Results:

All 55 E. coli O157:H7 strains tested positive; 41 non-target strains tested negative.
ANSR method performance was statistically equivalent to reference methods across all matrices.
Modest parameter changes did not impact assay performance.

Conclusions:

The modified ANSR method provides reliable detection of E. coli O157:H7.
The assay demonstrates robustness, stability, and a 1-year shelf-life.
Independent laboratory results confirmed the method's efficacy for food safety applications.