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Related Concept Videos

CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

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CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
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CRISPR/Cas9 Genome Editing01:28

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Targeted Cancer Therapies02:57

Targeted Cancer Therapies

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The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
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Targets for Drug Action: Overview01:26

Targets for Drug Action: Overview

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Drugs target macromolecules to modify ongoing cellular processes. Primary drug targets include receptors, ion channels, transporters, and enzymes.
Receptors are either membrane-spanning or intracellular proteins, which upon binding a ligand, get activated and transmit the signal downstream to elicit a response. Drugs bind receptors, either mimicking the action of endogenous ligands or blocking the receptor activity to bring about a modified response. Nearly 35% of approved drugs target the G...
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Related Experiment Video

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The multiplexed CRISPR targeting platforms.

Jian Cao1, Qian Xiao2, Qin Yan1

  • 1Department of Pathology, Yale School of Medicine, New Haven, CT 06520, United States.

Drug Discovery Today. Technologies
|September 13, 2018
PubMed
Summary
This summary is machine-generated.

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) technology offers advanced gene editing with advantages over ZFNs and TALENs. This review explores CRISPR strategies for efficient multiplexed gene targeting in biomedical research.

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Area of Science:

  • Biotechnology
  • Genomics
  • Molecular Biology

Background:

  • CRISPR technology has rapidly advanced biomedical research.
  • CRISPR offers significant advantages over older genome editing tools like ZFNs and TALENs.
  • Its potential in disease detection, prevention, and treatment is substantial.

Purpose of the Study:

  • To review the principles, approaches, and applications of CRISPR-based multiplexed gene targeting.
  • To highlight the advantages of CRISPR systems for simultaneous targeting of multiple genes.

Main Methods:

  • Discussion of various strategies and toolboxes developed for multiplexed CRISPR targeting.
  • Comparative analysis of CRISPR systems against ZFNs and TALENs.

Main Results:

  • CRISPR systems enable efficient simultaneous targeting of multiple genes.
  • Multiple strategies and toolboxes facilitate multiplexed gene targeting applications.

Conclusions:

  • CRISPR technology is a revolutionary tool in biomedical research.
  • The development of multiplexed targeting strategies enhances CRISPR's utility in advancing human disease research and treatment.