Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Adhesion01:14

Adhesion

44.5K
Adhesion occurs when one type of molecule is attracted to a different molecule. Water exhibits adhesive properties in the presence of polar surfaces, such as glass or cellulose in plants. For instance, when water is poured into a glass, the positively charged hydrogen molecules of water are more attracted to the negatively charged oxygen molecules in the silica than to the oxygen in neighboring water molecules.
Capillary action is a result of water’s adhesive tendencies. When a narrow...
44.5K
Master Transcription Regulators02:23

Master Transcription Regulators

7.8K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.8K
Epigenetic Regulation01:46

Epigenetic Regulation

33.8K
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
33.8K
GTPases and their Regulation02:14

GTPases and their Regulation

9.9K
Guanine nucleotide-binding proteins (G-proteins), also known as GTPases, are a superfamily of proteins that regulate many cellular processes, such as cell signaling, vesicular transport, and the regulation of cell shape and motility. Mutation or dysfunction of these proteins can lead to disease. There are around 40,000 known G-proteins that can broadly be classified into two groups ‒  small G-proteins consisting of a single domain and large multi-domain G-proteins.
Large G-proteins,...
9.9K
Regulated Protein Degradation02:58

Regulated Protein Degradation

8.9K
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
8.9K
Positive Regulator Molecules01:45

Positive Regulator Molecules

136.4K
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
136.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

IFITM1 and IFITM3 cooperate to restrict virus entry in endolysosomes.

Journal of virology·2026
Same author

Thrombin-PAR1 signaling regulates Topoisomerase 1 activity to bridge inflammation and breast cancer progression.

Cancer letters·2026
Same author

Spatial gradients of lipase activity govern lipid droplet remodeling in the zebrafish embryo.

Biochimica et biophysica acta. Molecular and cell biology of lipids·2026
Same author

The BHARAT study: a multi-modal, multi-omics investigation of aging signatures in the Indian population.

Aging·2026
Same author

Sphingosine-1-phosphate promotes CD8 T cell exhaustion in breast cancer via exosomal transfer of TGFBR2.

Cell death & disease·2026
Same author

Lipid biomarkers for differentiating asthma-COPD overlap: Insights from LC-MS-based lipidomics.

Respiratory medicine·2026

Related Experiment Video

Updated: Feb 5, 2026

Induction of Adhesion-dependent Signals Using Low-intensity Ultrasound
08:51

Induction of Adhesion-dependent Signals Using Low-intensity Ultrasound

Published on: May 8, 2012

9.9K

3D micro-environment regulates NF-κβ dependent adhesion to induce monocyte differentiation.

Anindita Bhattacharya1, Mahesh Agarwal1, Rachita Mukherjee1

  • 1School of Biological Science, Indian Association for the Cultivation of Science, Jadavpur, Kolkata, 700032, India.

Cell Death & Disease
|September 13, 2018
PubMed
Summary

Monocyte differentiation is driven by physical micro-environment changes. Cell adhesion in 2D or 3D environments triggers a signaling cascade, necessary and sufficient for differentiation.

More Related Videos

Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes
11:52

Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes

Published on: January 27, 2023

4.5K
Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages
06:46

Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages

Published on: June 30, 2016

31.0K

Related Experiment Videos

Last Updated: Feb 5, 2026

Induction of Adhesion-dependent Signals Using Low-intensity Ultrasound
08:51

Induction of Adhesion-dependent Signals Using Low-intensity Ultrasound

Published on: May 8, 2012

9.9K
Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes
11:52

Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes

Published on: January 27, 2023

4.5K
Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages
06:46

Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages

Published on: June 30, 2016

31.0K

Area of Science:

  • Cell Biology
  • Biophysics
  • Immunology

Background:

  • Monocyte differentiation involves migration from blood to tissues, altering their micro-environment.
  • While biochemical cues are understood, the physical transition from fluid-like to gel-like environments' role in differentiation is unclear.
  • Monocytes typically remain non-adherent to prevent differentiation.

Purpose of the Study:

  • To investigate the role of the physical micro-environment in monocyte differentiation.
  • To elucidate the signaling pathways involved in monocyte differentiation induced by micro-environmental changes.
  • To determine if cell adhesion is a critical factor in monocyte differentiation.

Main Methods:

  • Utilized 2D and 3D micro-environments with varying chemical compositions.
  • Monitored monocyte adhesion and activation of signaling pathways, including MAPK and NF-κβ.
  • Investigated the effects of chemical inducers in fluid-like micro-environments.

Main Results:

  • A 3D gel-like micro-environment induces monocyte differentiation via adhesion-MAPK-NF-κβ signaling, regardless of chemical makeup.
  • In 2D fluid-like environments, adhesion alone triggers the same differentiation pathway.
  • Chemical inducers enhance monocyte adhesion through p-MAPK signaling, leading to differentiation.
  • Established cell adhesion as both necessary and sufficient for monocyte differentiation in 2D/3D settings.

Conclusions:

  • Cell adhesion is a critical trigger for monocyte differentiation, mediated by MAPK and NF-κβ signaling.
  • The physical properties of the micro-environment, particularly its gel-like state, are potent drivers of monocyte differentiation.
  • Hypothesize that inert 3D gel-like environments influence other cellular functions due to the involvement of MAPK and NF-κβ in multiple pathways.