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Naphthol Blue Black and

Ji Youn Lee1,2, Ho Young Kim1, Yun-Sang Lee1

  • 1Department of Nuclear Medicine, Institute of Radiation Medicine, Seoul National University College of Medicine, Seoul, Republic of Korea.

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|September 13, 2018
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This study developed a novel multimodal radio-nanocarrier, 99mTc-MSA-NBB, for sentinel lymph node (SLN) mapping. The agent demonstrated excellent accumulation in lymph nodes, confirming its potential for enhanced SLN imaging.

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Area of Science:

  • Nuclear Medicine
  • Nanotechnology
  • Bioconjugation Chemistry

Background:

  • Sentinel lymph node (SLN) mapping is crucial for cancer staging.
  • Mannosylated human serum albumin (MSA) labeled with 99mTc is a known SLN imaging agent.
  • Multimodal imaging agents offer improved diagnostic capabilities.

Purpose of the Study:

  • To develop a novel multimodal radio-nanocarrier for SLN imaging.
  • To conjugate 99mTc-labeled MSA with a blue dye for visual, fluorescence, and SPECT/CT imaging.
  • To evaluate the efficacy of the developed conjugate for SLN mapping.

Main Methods:

  • Conjugation of seven blue dyes to MSA and assessment of binding affinities.
  • Selection of naphthol blue black (NBB) as the optimal dye based on spectroscopic and visual studies.
  • Preparation and in vivo evaluation of the 99mTc-MSA-NBB conjugate in mice following footpad injection.

Main Results:

  • The 99mTc-MSA-NBB conjugate exhibited high accumulation in popliteal lymph nodes within 1 hour post-injection.
  • SPECT/CT imaging revealed significant uptake in popliteal and inguinal lymph nodes from 10 minutes to 2 hours.
  • The multimodal agent facilitated SLN mapping through combined imaging modalities.

Conclusions:

  • A novel multimodal radio-nanocarrier, 99mTc-MSA-NBB, was successfully prepared.
  • The conjugate demonstrated excellent performance as a multimodal probe for sentinel lymph node mapping.
  • This agent holds promise for enhanced visualization and diagnosis in SLN imaging.