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Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

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Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
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Atomic Structure01:33

Atomic Structure

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Overview
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Atomic Structure01:17

Atomic Structure

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The Greek philosopher Democritus proposed that everything on Earth is made up of tiny particles called atomos, Greek for "indivisible," from which the modern term "atom" is derived. In the 19th century, John Dalton proposed the atomic theory that is still largely correct today. He put forth five postulates to explain how atoms made up the world around us. (1) All matter is composed of infinitely small particles or atoms. (2) All atoms of a given element are identical to one...
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Internal and External Forces01:12

Internal and External Forces

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Newton's first law states that a net external force causes a change in motion. External forces act on an object or system, originating outside of the object or system. In contrast, internal forces originate inside the system of interest and do not lead to any acceleration. In simpler words, internal forces are forces that act on one part of an object and are exerted by another part of the same object. External forces are forces that act on an object due to some other object. Therefore, when...
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Internal Forces and Center of Gravity01:25

Internal Forces and Center of Gravity

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Internal forces and the center of gravity are fundamental concepts in mechanics, playing a crucial role in understanding the behavior and stability of structures and objects under various conditions. A comprehensive understanding of these principles is essential for engineers, architects, and designers to create safe and efficient systems.
Internal forces are generated within a body due to the interaction between its particles. These forces can be categorized into tension, compression, and...
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Electronic Structure of Atoms02:28

Electronic Structure of Atoms

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An atom comprises protons and neutrons, which are contained inside the dense, central core called the nucleus, with electrons present around the nucleus. Taking into account the wave–particle duality of electrons and the uncertainty in position around the nucleus, quantum mechanics provides a more accurate model for the atomic structure. It describes atomic orbitals as the regions around the nucleus where electrons of discrete energy exist, characterized by four quantum...
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Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy
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Visualization of internal in situ cell structure by atomic force microscopy.

María L Segura-Valdez1, Lourdes T Agredano-Moreno1, Alma L Zamora-Cura1

  • 1Department of Cell Biology, Faculty of Sciences, National Autonomous University of Mexico (UNAM), Circuito Exterior, Ciudad Universitaria, Cd. Mx., Coyoacán, 04510, Mexico City, Mexico.

Histochemistry and Cell Biology
|September 13, 2018
PubMed
Summary

Atomic force microscopy (AFM) offers enhanced nanoscale resolution for in situ cell structure analysis. New sample preparation techniques enable detailed imaging of organelles within cells, surpassing light microscopy capabilities.

Keywords:
Atomic force microscopyCellNucleolusNucleusRNAUltramicrotome

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Area of Science:

  • Cell Biology
  • Nanotechnology
  • Microscopy

Background:

  • Light and electron microscopy are established cell structure analysis tools.
  • Atomic force microscopy (AFM) is a newer technique with potential for cell analysis.
  • Sample preparation challenges have limited AFM's application to in situ cell structures.

Purpose of the Study:

  • To review advancements in atomic force microscopy for in situ cell structure analysis.
  • To discuss methods for preparing samples for AFM imaging of cells.
  • To highlight the enhanced resolution achievable with AFM for nanoscale organelle structures.

Main Methods:

  • Review of existing literature on atomic force microscopy techniques for cell analysis.
  • Focus on sample preparation methods for in situ imaging, including resin embedding and ultramicrotomy.
  • Analysis of different AFM imaging modes applied to cell surfaces and sections.

Main Results:

  • AFM can image isolated molecules, organelles, and whole cells.
  • Emerging techniques allow for the analysis of in situ cell structures using AFM.
  • Imaging of semithin sections provides enhanced nanoscale resolution of organelle fine structures.

Conclusions:

  • Atomic force microscopy provides superior resolution for in situ cell structure analysis compared to light microscopy.
  • Advancements in sample preparation are crucial for unlocking AFM's potential in cell biology.
  • AFM enables detailed nanoscale investigation of organelle structures within their cellular context.