Related Experiment Video
Updated: Feb 5, 2026

Establishment of a Primary Culture of Patient-derived Soft Tissue Sarcoma
Published on: April 11, 2018
Mistaken identity: A Case for Karyotype Analysis Work-up of Soft Tissue Tumors
Justin Rueckert1, Alexandra Kalof1, Katherine Devitt1
1Department of Pathology and Laboratory Medicine, University of Vermont Medical Center, Burlington, VT.
Objectives:
Soft tissue pathology encompasses a diverse range of benign and malignant soft tissue tumors. Definitive diagnosis is challenging due to the vast number of histologic subtypes (>100) and the potential for overlapping clinical, radiographic, histologic, and/or immunohistochemical features. Many institutions have moved away from cytogenetic analysis in the workup of soft tissue tumors; however, specific non-random cytogenetic abnormalities are characteristic of various tumor types and can reveal or confirm the diagnosis in challenging cases. We present a diagnostically challenging case of myxoid liposarcoma initially considered to be reactive in nature and only correctly diagnosed when karyotype analysis revealed the characteristic t(12;16)(q13;p11.2), thus altering patient care and management.
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